9H94

Poliovirus type 2 (strain MEF-1) stabilised virus-like particle (PV2 SC5a) from a yeast expression system.

9H94 の概要

• エントリーDOI: 10.2210/pdb9h94/pdb
• EMDBエントリー: 51952
• 分子名称: Capsid protein VP1, Capsid protein VP0, Capsid protein VP3, ... (5 entities in total)
• 機能のキーワード: capsid protein, virus-like particle, vaccine, virus like particle
• 由来する生物種: Poliovirus 2; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 97320.93

構造登録者

Bahar, M.W.,Sherry, L.,Stonehouse, N.J.,Rowlands, D.J.,Fry, E.E.,Stuart, D.I. (登録日: 2024-10-29, 公開日: 2025-04-02, 最終更新日: 2025-04-09)

主引用文献

Sherry, L.,Grehan, K.,Bahar, M.W.,Swanson, J.J.,Fox, H.,Matthews, S.,Carlyle, S.,Qin, L.,Porta, C.,Wilkinson, S.,Robb, S.,Clark, N.,Liddell, J.,Fry, E.E.,Stuart, D.I.,Macadam, A.J.,Rowlands, D.J.,Stonehouse, N.J.
Production of an immunogenic trivalent poliovirus virus-like particle vaccine candidate in yeast using controlled fermentation.
Npj Vaccines, 10:64-64, 2025

PubMed Abstract: The success of the poliovirus (PV) vaccines has enabled the near-eradication of wild PV, however, their continued use post-eradication poses concerns, due to the potential for virus escape during vaccine manufacture. Recombinant virus-like particles (VLPs) that lack the viral genome remove this risk. Here, we demonstrate the production of PV VLPs for all three serotypes by controlled fermentation using Pichia pastoris. We determined the cryo-EM structure of a new PV2 mutant, termed SC5a, in comparison to PV2-SC6b VLPs described previously and investigated the immunogenicity of PV2-SC5a VLPs. Finally, a trivalent immunogenicity trial using bioreactor-derived VLPs of all three serotypes in the presence of Alhydrogel adjuvant, showed that these VLPs outperform the current IPV vaccine in the standard vaccine potency assay, offering the potential for dose-sparing. Overall, these results provide further evidence that yeast-produced VLPs have the potential to be a next-generation polio vaccine in a post-eradication world.

PubMed: 40164627
DOI: 10.1038/s41541-025-01111-2

実験手法

ELECTRON MICROSCOPY (2.1 Å)