9H3H

Human Carbonic anhydrase II crystallization and structure determination at room temperature in the CrystalChip

Summary for 9H3H

• Entry DOI: 10.2210/pdb9h3h/pdb
• Descriptor: Carbonic anhydrase 2, ZINC ION (3 entities in total)
• Functional Keywords: human carbonic anhydrase ii, microfluidics, room temperature, lyase
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 28998.05

Authors

Pachl, P.,Coudray, L.,Rezacova, P.,Fejfarova, A.,Vincent, R.,Engilberge, S.,Sauter, C. (deposition date: 2024-10-16, release date: 2024-12-04)

Primary citation

Pachl, P.,Coudray, L.,Vincent, R.,Nilles, L.,Scheer, H.,Ritzenthaler, C.,Fejfarova, A.,Rezacova, P.,Engilberge, S.,Sauter, C.
Protein crystallization and structure determination at room temperature in the CrystalChip.
Febs Open Bio, 2024

PubMed Abstract: The production of high-quality crystals is a key step in crystallography in general, but control of crystallization conditions is even more crucial in serial crystallography, which requires sets of crystals homogeneous in size and diffraction properties. This protocol describes the implementation of a simple and user-friendly microfluidic device that allows both the production of crystals by the counter-diffusion method and their in situ analysis by serial crystallography. As an illustration, the whole procedure is used to determine the crystal structure of three proteins from data collected at room temperature at a synchrotron radiation source.

PubMed: 39572886
DOI: 10.1002/2211-5463.13932

Experimental method

X-RAY DIFFRACTION (1.8 Å)