9H3E

Hen egg white lysozyme crystallization and structure determination at room temperature in the CrystalChip

9H3E の概要

• エントリーDOI: 10.2210/pdb9h3e/pdb
• 分子名称: Lysozyme C, SODIUM ION, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: lysozyme, microfluidics, room temperature, hydrolase
• 由来する生物種: Gallus gallus (chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16387.01

構造登録者

Pachl, P.,Coudray, L.,VIncent, R.,Sauter, C. (登録日: 2024-10-16, 公開日: 2024-12-04, 最終更新日: 2025-04-16)

主引用文献

Pachl, P.,Coudray, L.,Vincent, R.,Nilles, L.,Scheer, H.,Ritzenthaler, C.,Fejfarova, A.,Rezacova, P.,Engilberge, S.,Sauter, C.
Protein crystallization and structure determination at room temperature in the CrystalChip.
Febs Open Bio, 15:532-541, 2025

PubMed Abstract: The production of high-quality crystals is a key step in crystallography in general, but control of crystallization conditions is even more crucial in serial crystallography, which requires sets of crystals homogeneous in size and diffraction properties. This protocol describes the implementation of a simple and user-friendly microfluidic device that allows both the production of crystals by the counter-diffusion method and their in situ analysis by serial crystallography. As an illustration, the whole procedure is used to determine the crystal structure of three proteins from data collected at room temperature at a synchrotron radiation source.

PubMed: 39572886
DOI: 10.1002/2211-5463.13932

実験手法

X-RAY DIFFRACTION (1.5 Å)