9H2A

AcMNPV complete basal cap

9H2A の概要

• エントリーDOI: 10.2210/pdb9h2a/pdb
• 関連するPDBエントリー: 9H1S
• EMDBエントリー: 51791
• 分子名称: Occlusion-derived virus envelope protein E27, Protein C42, Uncharacterized 38.0 kDa protein in P143-LEF5 intergenic region, ... (8 entities in total)
• 機能のキーワード: nucleocapsid, virus
• 由来する生物種: Autographa californica nucleopolyhedrovirus; 詳細
• タンパク質・核酸の鎖数: 32
• 化学式量合計: 1520015.11

構造登録者

Effantin, G.,Kandiah, E.,Pelosse, M. (登録日: 2024-10-11, 公開日: 2025-06-04)

主引用文献

Effantin, G.,Kandiah, E.,Pelosse, M.
Structure of AcMNPV nucleocapsid reveals DNA portal organization and packaging apparatus of circular dsDNA baculovirus.
Nat Commun, 16:4844-4844, 2025

PubMed Abstract: Baculoviruses are large DNA viruses found in nature propagating amongst insects and lepidoptera in particular. They have been studied for decades and are nowadays considered as invaluable biotechnology tools used as biopesticides, recombinant expression systems or delivery vehicle for gene therapy. However, little is known about the baculovirus nucleocapsid assembly at a molecular level. Here, we solve the whole structure of the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) nucleocapsid by applying cryo-electron microscopy (CryoEM) combined with de novo modelling and Alphafold predictions. Our structure completes prior observations and elucidates the intricate architecture of the apical cap, unravelling the organization of a DNA portal featuring intriguing symmetry mismatches between its core and vertex. The core, closing the capsid at the apex, holds two DNA helices of the viral genome tethered to Ac54 proteins. Different symmetry components at the apical cap and basal structure are constituted of the same building block, made of Ac101/Ac144, proving the versatility of this modular pair. The crown forming the portal vertex displays a C21 symmetry and contains, amongst others, the motor-like protein Ac66. Our findings support the viral portal to be involved in DNA packaging, probably in conjunction with other parts of a larger DNA packaging apparatus.

PubMed: 40413174
DOI: 10.1038/s41467-025-60152-2

実験手法

ELECTRON MICROSCOPY (5.2 Å)