9GUR

30S mRNA delivery complex TEC resolved (TEC only)

9GUR の概要

• エントリーDOI: 10.2210/pdb9gur/pdb
• EMDBエントリー: 51617
• 分子名称: DNA-directed RNA polymerase subunit alpha, MAGNESIUM ION, ZINC ION, ... (11 entities in total)
• 機能のキーワード: transcription, translation, coupling, ribosome
• 由来する生物種: Escherichia coli K-12; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 396945.32

構造登録者

Rahil, H.,Weixlbaumer, A.,Webster, M.W. (登録日: 2024-09-20, 公開日: 2024-11-20, 最終更新日: 2025-07-02)

主引用文献

Webster, M.W.,Chauvier, A.,Rahil, H.,Graziadei, A.,Charles, K.,Miropolskaya, N.,Takacs, M.,Saint-Andre, C.,Rappsilber, J.,Walter, N.G.,Weixlbaumer, A.
Molecular basis of mRNA delivery to the bacterial ribosome.
Science, 386:eado8476-eado8476, 2024

PubMed Abstract: Protein synthesis begins with the formation of a ribosome-messenger RNA (mRNA) complex. In bacteria, the small ribosomal subunit (30) is recruited to many mRNAs through base pairing with the Shine-Dalgarno (SD) sequence and RNA binding by ribosomal protein bS1. Translation can initiate on nascent mRNAs, and RNA polymerase (RNAP) can promote the recruitment of the pioneering 30. Here, we examined 30 recruitment to nascent mRNAs using cryo-electron microscopy, single-molecule fluorescence colocalization, and in-cell cross-linking mass spectrometry. We show that bS1 delivers the mRNA to the ribosome for SD duplex formation and 30 activation. Additionally, bS1 and RNAP stimulate translation initiation. Our work provides a mechanistic framework for how the SD duplex, ribosomal proteins, and RNAP cooperate in 30 recruitment to mRNAs and establish transcription-translation coupling.

PubMed: 39607923
DOI: 10.1126/science.ado8476

実験手法

ELECTRON MICROSCOPY (4.2 Å)