9GSX

Campylobacter hook-filament junction-cap complex

これはPDB形式変換不可エントリーです。

9GSX の概要

• エントリーDOI: 10.2210/pdb9gsx/pdb
• EMDBエントリー: 51557
• 分子名称: Flagellin, Flagellar hook-associated protein 1, Flagellar hook-associated protein 2 (3 entities in total)
• 機能のキーワード: campylobacter jejuni, flagellar cap, hook-filament junction, flid, flgk, flgl, flagella, transport protein
• 由来する生物種: Campylobacter jejuni; 詳細
• タンパク質・核酸の鎖数: 27
• 化学式量合計: 1990072.42

構造登録者

Qin, K.,Gonzalez-Rodriguez, N.,Shmakova, E.,Beeby, M.,Bergeron, J.R.C. (登録日: 2024-09-16, 公開日: 2025-04-09, 最終更新日: 2025-07-16)

主引用文献

Einenkel, R.,Qin, K.,Schmidt, J.,Al-Otaibi, N.S.,Mann, D.,Drobnic, T.,Cohen, E.J.,Gonzalez-Rodriguez, N.,Harrowell, J.,Shmakova, E.,Beeby, M.,Erhardt, M.,Bergeron, J.R.C.
The structure of the complete extracellular bacterial flagellum reveals the mechanism of flagellin incorporation.
Nat Microbiol, 10:1741-1757, 2025

PubMed Abstract: The bacterial flagellum is essential for motility, adhesion and colonization in pathogens such as Salmonella enterica and Campylobacter jejuni. Its extracellular structure comprises the hook, hook-filament junction, filament and filament cap. Native structures of the hook-filament junction and the cap are lacking, and molecular mechanisms of cap-mediated filament assembly are largely uncharacterized. Here we use cryo-electron microscopy to resolve structures of the complete Salmonella extracellular flagellum including the pentameric FliD cap complex (3.7 Å) and the FlgKL hook-filament junction (2.9 Å), as well as the Campylobacter extracellular flagellum before filament assembly (6.5 Å). This, coupled with structure-guided mutagenesis and functional assays, reveals intermediates of filament assembly, showing that FliD cap protein terminal domain movement and clockwise rotation enable flagellin incorporation and stabilization of the filament. We show that the hook-filament junction acts as a buffer, preventing transfer of mechanical stress to the filament, and reveal the structural basis for the initiation of filament assembly. Collectively, this study provides comprehensive insights into flagellum assembly and how flagellin incorporation is coupled with its secretion.

PubMed: 40595287
DOI: 10.1038/s41564-025-02037-0

実験手法

ELECTRON MICROSCOPY (6.5 Å)