9GOK

X-ray structure of lysozyme obtained upon reaction with the dioxidovanadium(V) complex with (E)-N'-(1-(2-hydroxy-5-methoxyphenyl)ethylidene)furan-2-carbohydrazide)

これはPDB形式変換不可エントリーです。

9GOK の概要

• エントリーDOI: 10.2210/pdb9gok/pdb
• 分子名称: Lysozyme C, SODIUM ION, ACETATE ION, ... (6 entities in total)
• 機能のキーワード: metallodrugs, protein metalation, vanadium compounds, protein metal compounds interactions, hydrolase
• 由来する生物種: Gallus gallus (chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15288.99

構造登録者

Paolillo, M.,Ferraro, G.,Merlino, A. (登録日: 2024-09-05, 公開日: 2024-12-18)

主引用文献

Paolillo, M.,Ferraro, G.,Sahu, G.,Pattanayak, P.D.,Garribba, E.,Halder, S.,Ghosh, R.,Mondal, B.,Chatterjee, P.B.,Dinda, R.,Merlino, A.
Interaction of V V O 2 -hydrazonates with lysozyme.
J.Inorg.Biochem., 264:112787-112787, 2024

PubMed Abstract: Vanadium compounds (VCs) exhibit a broad range of pharmacological properties, with their most significant medical applications being in the treatment of cancer and diabetes. The therapeutic effects and mode of action of VCs may be associated with their ability to bind proteins and, consequently, understanding the VC-protein interaction is of paramount importance. Among the promising VCs, the VO complex with the aroylhydrazone furan-2-carboxylic acid ((3-ethoxy-2-hydroxybenzylidene)hydrazide, hereafter denoted as VC1), deserves attention, since it exhibits cytotoxicity against various cancer cell lines, including HeLa. The interaction between VC1 and its analogue, denoted as VC2 (the dioxidovanadium(V) complex with (E)-N'-(1-(2-hydroxy-5-methoxyphenyl)ethylidene)furan-2-carbohydrazide), and hen egg white lysozyme (HEWL) was examined by UV-vis spectroscopy, fluorescence, circular dichroism, and X-ray crystallography. The interaction of VC1 and VC2 with HEWL does not alter the protein secondary and tertiary structure. Crystallographic studies indicate that the two metal complexes or V-containing fragments originating from VC1 and VC2 bind the protein via non-covalent interactions. Furthermore, when bound to HEWL, two VC1 molecules and two VC2 molecules form a supramolecular association stabilized by stacking interactions. This type of interaction could favour the binding of similar compounds to proteins and affect their biological activity.

PubMed: 39642703
DOI: 10.1016/j.jinorgbio.2024.112787

実験手法

X-RAY DIFFRACTION (1.18 Å)