9GOE

Cryo-EM structure of the multiple peptide resistance factor (MprF) from Pseudomonas aeruginosa bound to a synthetic nanobody (Sb29)

9GOE の概要

• エントリーDOI: 10.2210/pdb9goe/pdb
• EMDBエントリー: 51497
• 分子名称: Phosphatidylglycerol lysyltransferase, Synthetic nanobody (Sybody) 29 (2 entities in total)
• 機能のキーワード: lipid transport, sybody complex, antimicrobial resistance, saposin-protein nanoparticle, membrane protein
• 由来する生物種: Pseudomonas aeruginosa PAO1; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 112892.57

構造登録者

Hankins, M.T.K.,Parrag, M.,Garaeva, A.A.,Earp, J.C.,Seeger, M.A.,Stansfeld, P.J.,Bublitz, M. (登録日: 2024-09-05, 公開日: 2025-03-12, 最終更新日: 2025-04-23)

主引用文献

Hankins, M.T.K.,Parrag, M.,Garaeva, A.A.,Earp, J.C.,Seeger, M.A.,Stansfeld, P.J.,Bublitz, M.
MprF from Pseudomonas aeruginosa is a promiscuous lipid scramblase with broad substrate specificity.
Sci Adv, 11:eads9135-eads9135, 2025

PubMed Abstract: The multiple peptide resistance factor (MprF) is a bifunctional membrane protein found in many bacteria, including and . MprF modifies inner leaflet lipid headgroups through aminoacylation and translocates modified lipid to the outer leaflet. This activity provides increased resistance to antimicrobial agents. MprF presents a promising target in multiresistant pathogens, but structural information is limited and both substrate specificity and energization of MprF-mediated lipid transport are poorly understood. Here, we present the cryo-EM structure of MprF from (MprF) bound to a synthetic nanobody. MprF adopts an "open" conformation with a wide, lipid-exposed groove on the periplasmic side that induces a local membrane deformation in molecular dynamics simulations. Using an in vitro liposome transport assay, we demonstrate that MprF translocates a wide range of different lipids without an external energy source. This suggests that MprF is the first dedicated lipid scramblase to be characterized in bacteria.

PubMed: 40203087
DOI: 10.1126/sciadv.ads9135

実験手法

ELECTRON MICROSCOPY (3.28 Å)