9GN2

Nucleoside-2'-deoxyribosyltransferase from Lactobacillus leichmannii. Complex with ribose and cytosine

9GN2 の概要

• エントリーDOI: 10.2210/pdb9gn2/pdb
• 分子名称: Nucleoside deoxyribosyltransferase, 6-AMINOPYRIMIDIN-2(1H)-ONE, alpha-D-ribofuranose, ... (4 entities in total)
• 機能のキーワード: nucleoside-2'-deoxyribosyltransferase, transferase
• 由来する生物種: Lactobacillus leichmannii
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 36457.94

構造登録者

Ascham, A.,Salihovic, A.,Burley, G.,Grogan, G. (登録日: 2024-08-30, 公開日: 2024-12-25, 最終更新日: 2025-01-29)

主引用文献

Salihovic, A.,Ascham, A.,Rosenqvist, P.S.,Taladriz-Sender, A.,Hoskisson, P.A.,Hodgson, D.R.W.,Grogan, G.,Burley, G.A.
Biocatalytic synthesis of ribonucleoside analogues using nucleoside transglycosylase-2.
Chem Sci, 16:1302-1307, 2025

PubMed Abstract: Ribonucleosides are essential building blocks used extensively in antiviral and oligonucleotide therapeutics. A major challenge in the further development of nucleoside analogues for therapeutic applications is access to scalable and environmentally sustainable synthetic strategies. This study uses the type II nucleoside 2'-deoxyribosyltransferase from (NDT-2) to prepare a suite of ribonucleoside analogues using naturally-occurring uridine and cytidine sugar donors. Crystal structure and mutational analyses are used to define the substrate tolerance of the nucleobase exchange and the 2'-substituent of the nucleoside sugar donor. Nucleobase profiling identified acceptance of both purine and pyrimidine nucleobases. Finally, the scalability of the approach is showcased, enabling the preparation of ribonucleosides on millimolar scales. This biocatalytic strategy opens up opportunities to establish chemoenzymatic routes to prepare nucleoside analogues incorporating 2' modifications that are of therapeutic importance.

PubMed: 39691463
DOI: 10.1039/d4sc07521h

実験手法

X-RAY DIFFRACTION (2.41 Å)