9GM5

OCCM maturation intermediate stalled with an Arginine Finger mutation in Mcm5: Conformer 1

これはPDB形式変換不可エントリーです。

9GM5 の概要

• エントリーDOI: 10.2210/pdb9gm5/pdb
• EMDBエントリー: 51441
• 分子名称: DNA replication licensing factor MCM2, Origin recognition complex subunit 3, Origin recognition complex subunit 4, ... (17 entities in total)
• 機能のキーワード: aaa+ atpase, dna translocase, atpase switch, dna replication, replication
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast); 詳細
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 1119438.59

構造登録者

Butryn, A.,Costa, A. (登録日: 2024-08-28, 公開日: 2025-03-05)

主引用文献

Butryn, A.,Greiwe, J.F.,Costa, A.
Unidirectional MCM translocation away from ORC drives origin licensing.
Nat Commun, 16:782-782, 2025

PubMed Abstract: The MCM motor of the eukaryotic replicative helicase is loaded as a double hexamer onto DNA by the Origin Recognition Complex (ORC), Cdc6, and Cdt1. ATP binding supports formation of the ORC-Cdc6-Cdt1-MCM (OCCM) helicase-recruitment complex where ORC-Cdc6 and one MCM hexamer form two juxtaposed rings around duplex DNA. ATP hydrolysis by MCM completes MCM loading but the mechanism is unknown. Here, we used cryo-EM to characterise helicase loading with ATPase-dead Arginine Finger variants of the six MCM subunits. We report the structure of two MCM complexes with different DNA grips, stalled as they mature to loaded MCM. The Mcm2 Arginine Finger-variant stabilises DNA binding by Mcm2 away from ORC/Cdc6. The Arginine Finger-variant of the neighbouring Mcm5 subunit stabilises DNA engagement by Mcm5 downstream of the Mcm2 binding site. Cdc6 and Orc1 progressively disengage from ORC as MCM translocates along DNA. We observe that duplex DNA translocation by MCM involves a set of leading-strand contacts by the pre-sensor 1 ATPase hairpins and lagging-strand contacts by the helix-2-insert hairpins. Mutating any of the MCM residues involved impairs high-salt resistant DNA binding in vitro and double-hexamer formation assessed by electron microscopy. Thus, ATPase-powered duplex DNA translocation away from ORC underlies MCM loading.

PubMed: 39824870
DOI: 10.1038/s41467-025-56143-y

実験手法

ELECTRON MICROSCOPY (3.7 Å)