9GLG

X-ray structure of the Thermus thermophilus Q218E mutant of the PilF-GSPIIB domain in the c-di-GMP bound state

9GLG の概要

• エントリーDOI: 10.2210/pdb9glg/pdb
• 分子名称: ATP-binding motif-containing protein pilF, 9,9'-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d:3',2'-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one), SULFATE ION, ... (5 entities in total)
• 機能のキーワード: gspii, c-di-gmp, ligand binding, motor protein
• 由来する生物種: Thermus thermophilus HB27
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 34712.44

構造登録者

Neissner, K.,Woehnert, J. (登録日: 2024-08-27, 公開日: 2025-06-25, 最終更新日: 2025-07-09)

主引用文献

Neissner, K.,Duchardt-Ferner, E.,Wiedemann, C.,Kraus, J.,Hellmich, U.A.,Wohnert, J.
Protonated Glutamate and Aspartate Side Chains Can Recognize Phosphodiester Groups via Strong and Short Hydrogen Bonds in Biomacromolecular Complexes.
Angew.Chem.Int.Ed.Engl., 64:e202501589-e202501589, 2025

PubMed Abstract: Phosphodiester groups occur ubiquitously in nature, e.g. in nucleic acids or in cyclic (di-)nucleotides important for signal transduction. Proteins often use polar or positively charged amino acids to interact with the negatively charged phosphodiester groups via hydrogen bonds and salt bridges. In contrast, the acidic amino acids aspartate and glutamate are generally not considered as important determinants for phosphodiester group recognition. Instead, they are regarded as detrimental to such interactions due to the assumed charge repulsion between their deprotonated, negatively charged side chain carboxylate groups and the phosphodiester. Accordingly, acidic amino acids are often purposefully introduced into proteins to abrogate nucleic acid interactions in functional studies. Here, we show that in appropriate structural contexts, glutamate side chains are readily protonated even at neutral pH and act as hydrogen bond donors to phosphodiester groups using a c-di-GMP binding protein - the GSPII-B domain of PilF from Thermus thermophilus - as an example. Surveying available RNA-protein and DNA-protein complex structures in the PDB, we found that hydrogen bonds between apparently protonated carboxylate groups of glutamate and aspartate and phosphodiester groups occur frequently in many different functional protein classes. Thus, the functional role of acidic amino acids in phosphodiester group recognition needs to be re-evaluated.

PubMed: 40272996
DOI: 10.1002/anie.202501589

実験手法

X-RAY DIFFRACTION (1.55 Å)