9GI7

Hen Egg-White Lysozyme (HEWL) complexed with a Lindqvist-type hexavanadate (V6-OH) polyoxometalate

これはPDB形式変換不可エントリーです。

9GI7 の概要

• エントリーDOI: 10.2210/pdb9gi7/pdb
• 分子名称: Lysozyme C, hexavanadate (V6-OH) polyoxometalate, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: complex, protein binding
• 由来する生物種: Gallus gallus (chicken)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 15157.03

構造登録者

Moussawi, M.A.,Parac-Vogt, T.N. (登録日: 2024-08-17, 公開日: 2025-01-29, 最終更新日: 2025-04-02)

主引用文献

Moussawi, M.A.,de Azambuja, F.,Parac-Vogt, T.N.
Discrete Hybrid Vanadium-oxo Cluster as a Targeted Tool for Selective Protein Oxidative Modifications and Cleavage.
Angew.Chem.Int.Ed.Engl., 64:e202423078-e202423078, 2025

PubMed Abstract: Understanding the impact of oxidative modification on protein structure and functions is essential for developing therapeutic strategies to combat macromolecular damage and cell death. However, selectively inducing oxidative modifications in proteins under physiological conditions remains challenging. Herein we demonstrate that [VO{(OCH)CCHOH}] (V-OH) hybrid metal-oxo cluster can be used for selective protein oxidative cleavage and modifications. We present the first example of a protein-bound hybrid vanadate cluster, where its interactions with protein surfaces and the redox activity of vanadium enable selective oxidative modifications. Single Crystal X-ray Diffraction (SC-XRD) of the V-OH and hen egg white lysozyme (HEWL) complex revealed that the binding is dictated both by the inorganic core and the organic ligands attached to it. Selective oxidation or cleavage of HEWL occurs under physiological conditions by producing reactive oxygen species (ROS) in presence of ascorbate (Asc) as a reducing agent. The outcome of the oxidative reaction can be tuned by varying the concentration of V-OH to result either in selective oxidation of the amino acid side chains or peptide bond cleavage. LC-MS and crystallography revealed that oxidative modifications were mainly concentrated near the cluster binding sites, providing spatial control of ROS production. This study advances our understanding of vanadium's role in biological systems and demonstrates the potential of hybrid metal-oxo clusters in protein modification.

PubMed: 39792069
DOI: 10.1002/anie.202423078

実験手法

X-RAY DIFFRACTION (1.76 Å)