9GEE

Entamoeba histolytica Gal/GalNAc lectin heavy chain residues 808-992 in the presence of galactose

9GEE の概要

• エントリーDOI: 10.2210/pdb9gee/pdb
• 分子名称: Galactose/N-acetyl-D-galactosamine lectin heavy subunit 1, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• 機能のキーワード: entamoeba histolytica, lectin, gal/galnac, trogocytosis, cell adhesion
• 由来する生物種: Entamoeba histolytica
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 65969.53

構造登録者

Gerard, S.F.,Higgins, M.K. (登録日: 2024-08-07, 公開日: 2026-02-11, 最終更新日: 2026-04-01)

主引用文献

Gerard, S.F.,Redfield, C.,Higgins, M.K.
Structural basis for carbohydrate recognition by the Gal/GalNAc lectin of Entamoeba histolytica involved in host cell adhesion.
Plos Pathog., 22:e1013948-e1013948, 2026

PubMed Abstract: Intestinal amoebiasis is caused by Entamoeba histolytica, one of the deadliest human-infective parasites. Central to its pathogenicity is its binding to mucosal carbohydrates, which precedes tissue damage by trogocytosis. Carbohydrate binding is mediated by a single adhesin, the galactose/N-acetylgalactosamine (Gal/GalNAc) lectin, which is the leading vaccine candidate for amoebiasis. We present the structure of the native heterodimeric lectin, revealing an ordered core containing the light chain and the N-terminal region of the heavy chain. Structures obtained in the presence of ligand show that the Gal/GalNAc binding site is in the light chain, which adopts a β-trefoil fold found in other lectins. An elongated arm emerges from the heavy chain, which adopts multiple positions and may be modulated by sugar binding. This study reveals the molecular basis for sugar binding by the Entamoeba histolytica Gal/GalNAc lectin, a prerequisite for parasite invasion and development of intestinal disease.

PubMed: 41734235
DOI: 10.1371/journal.ppat.1013948

実験手法

X-RAY DIFFRACTION (1.818 Å)