9GC3

yeast TFIIIC TauB subcomplex bound to a tRNA gene

9GC3 の概要

• エントリーDOI: 10.2210/pdb9gc3/pdb
• EMDBエントリー: 51228
• 分子名称: Transcription factor tau 138 kDa subunit, Transcription factor tau 91 kDa subunit, Transcription factor tau 60 kDa subunit, ... (5 entities in total)
• 機能のキーワード: base-specific contacts, b-box, trna gene, dna binding protein
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast); 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 305466.58

構造登録者

Seifert-Davila, W.,Girbig, M.,Hauptmann, L.,Hoffmann, T.,Eustermann, S.,Mueller, C.,Chaban, A.,Duss, O.,Baudin, F. (登録日: 2024-08-01, 公開日: 2024-11-27, 最終更新日: 2025-01-22)

主引用文献

Seifert-Davila, W.,Chaban, A.,Baudin, F.,Girbig, M.,Hauptmann, L.,Hoffmann, T.,Duss, O.,Eustermann, S.,Muller, C.W.
Structural and kinetic insights into tRNA promoter engagement by yeast general transcription factor TFIIIC.
Nucleic Acids Res., 53:-, 2025

PubMed Abstract: Transcription of transfer RNA (tRNA) genes by RNA polymerase (Pol) III requires the general transcription factor IIIC (TFIIIC), which recognizes intragenic A-box and B-box DNA motifs of type II gene promoters. However, the underlying mechanism has remained elusive, in part due to missing structural information for A-box recognition. In this study, we use single-particle cryogenic electron microscopy (cryo-EM) and single-molecule fluorescence resonance energy transfer (smFRET) to reveal structural and real-time kinetic insights into how the 520-kDa yeast TFIIIC complex engages A-box and B-box DNA motifs in the context of a tRNA gene promoter. Cryo-EM structures of τA and τB subcomplexes bound to the A-box and B-box were obtained at 3.7 and 2.5 Å resolution, respectively, while cryo-EM single-particle mapping determined the specific distance and relative orientation of the τA and τB subcomplexes revealing a fully engaged state of TFIIIC. smFRET experiments show that overall recruitment and residence times of TFIIIC on a tRNA gene are primarily governed by B-box recognition, while footprinting experiments suggest a key role of τA and the A-box in TFIIIB and Pol III recruitment following TFIIIC recognition of type II promoters.

PubMed: 39657784
DOI: 10.1093/nar/gkae1174

実験手法

ELECTRON MICROSCOPY (2.46 Å)