9GAU

Sumo-Darpin-C10-complex

9GAU の概要

• エントリーDOI: 10.2210/pdb9gau/pdb
• 関連するPDBエントリー: 9G8I
• 分子名称: DARPin, Ubiquitin-like protein SMT3 (3 entities in total)
• 機能のキーワード: sumo, darpin, ankyrin repeats, signaling protein
• 由来する生物種: synthetic construct; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 22149.73

構造登録者

Wolf, E.,Cakilkaya, B.,Boergel, A. (登録日: 2024-07-29, 公開日: 2025-03-05, 最終更新日: 2025-03-12)

主引用文献

Troster, V.,Wong, R.P.,Borgel, A.,Cakilkaya, B.,Renz, C.,Mockel, M.M.,Eifler-Olivi, K.,Marinho, J.,Reinberg, T.,Furler, S.,Schaefer, J.V.,Pluckthun, A.,Wolf, E.,Ulrich, H.D.
Custom affinity probes reveal DNA-damage-induced, ssDNA-independent chromatin SUMOylation in budding yeast.
Cell Rep, 44:115353-115353, 2025

PubMed Abstract: The small ubiquitin-related modifier SUMO regulates cellular processes in eukaryotes either by modulating individual protein-protein interactions or with relaxed substrate selectivity by group modification. Here, we report the isolation and characterization of designed ankyrin repeat protein (DARPin)-based affinity probes directed against budding yeast SUMO (Smt3). We validate selected DARPins as compartment-specific inhibitors or neutral detection agents. Structural characterization reveals a recognition mode distinct from that of natural SUMO interactors. In vivo application pinpoints Smt3's essential function to the nucleus and demonstrates DARPin-mediated sensitization toward various stress conditions. A subset of selected clones is validated as SUMOylation reporters in cells. In this manner, we identify a DNA-damage-induced nuclear SUMOylation response that-in contrast to previously reported chromatin group SUMOylation-is independent of single-stranded DNA and the SUMO-E3 Siz2 but depends on Mms21 and likely reflects late intermediates of homologous recombination. Thus, Smt3-specific DARPins can provide insight into the dynamics of SUMOylation in defined subcellular structures.

PubMed: 40019834
DOI: 10.1016/j.celrep.2025.115353

実験手法

X-RAY DIFFRACTION (2.64 Å)