9GAP

CryoEM structure of influenza A RNP-like particle double-stranded assembled with a 12-mer RNA.

これはPDB形式変換不可エントリーです。

9GAP の概要

• エントリーDOI: 10.2210/pdb9gap/pdb
• EMDBエントリー: 51184 51185 51186 51187 51188 51189
• 分子名称: RNA (5'-P(UC)6-FAM3'), Nucleoprotein, 2-[3,6-bis(oxidanylidene)-4,5-dihydroxanthen-9-yl]-4-[3-[(2R)-2-oxidanylpropoxy]propylcarbamoyl]benzoic acid (3 entities in total)
• 機能のキーワード: nucleoprotein, rna, complex, rnp-like, influenza virus, viral protein
• 由来する生物種: Influenza A virus; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 120040.55

構造登録者

Chenavier, F.,Ruigrok, R.W.H.,Schoehn, G.,Ballandras-Colas, A.,Crepin, T. (登録日: 2024-07-29, 公開日: 2025-01-15, 最終更新日: 2025-02-12)

主引用文献

Chenavier, F.,Zarkadas, E.,Freslon, L.L.,Stelfox, A.J.,Schoehn, G.,Ruigrok, R.W.H.,Ballandras-Colas, A.,Crepin, T.
Influenza a virus antiparallel helical nucleocapsid-like pseudo-atomic structure.
Nucleic Acids Res., 53:-, 2025

PubMed Abstract: Influenza A viruses are responsible for human seasonal epidemics and severe animal pandemics with a risk of zoonotic transmission to humans. The viral segmented RNA genome is encapsidated by nucleoproteins (NP) and attached to the heterotrimeric polymerase, forming the viral ribonucleoproteins (vRNPs). Flexible helical vRNPs are central for viral transcription and replication. In this study, we present an advanced biological tool, the antiparallel helical RNP-like complex, assembled from recombinant N-terminally truncated NP and short synthetic RNA. The 3.0 Å cryo-electron microscopy structure details for the first time the whole RNA pathway across NP as well as NP-NP interactions that drive the antiparallel helical assembly accommodating major and minor grooves. Our findings show that the surface of the protein can harbour several conformations of the RNA, confirming that the number of nucleobases that binds to NP is not fixed, but ranges probably between 20 and 24. Taking all together, our data provide details to further understand the genome encapsidation and explain the inherent flexibility of influenza A virus vRNPs.

PubMed: 39673795
DOI: 10.1093/nar/gkae1211

実験手法

ELECTRON MICROSCOPY (4 Å)