9GA3

MtUvrA2UvrB bound to damaged oligonucleotide

これはPDB形式変換不可エントリーです。

9GA3 の概要

• エントリーDOI: 10.2210/pdb9ga3/pdb
• EMDBエントリー: 51170
• 分子名称: UvrABC system protein A, DNA, UvrABC system protein B, ... (5 entities in total)
• 機能のキーワード: dna repair, ner, uvra, uvrb, uvr, mtb, dna binding protein
• 由来する生物種: Mycobacterium tuberculosis; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 325374.98

構造登録者

Genta, M.,Capelli, R.,Ferrara, G.,Rizzi, M.,Rossi, F.,Jeruzalmi, D.,Bolognesi, M.,Chaves-Sanjuan, A.,Miggiano, R. (登録日: 2024-07-26, 公開日: 2025-04-23, 最終更新日: 2025-07-09)

主引用文献

Genta, M.,Ferrara, G.,Capelli, R.,Rondelli, D.,Sertic, S.,Bolognesi, M.,Rizzi, M.,Rossi, F.,Jeruzalmi, D.,Chaves-Sanjuan, A.,Miggiano, R.
Mechanistic understanding of UvrA damage detection and lesion hand-off to UvrB in Nucleotide Excision Repair.
Nat Commun, 16:3416-3416, 2025

PubMed Abstract: Nucleotide excision repair (NER) represents one of the major molecular machineries that control chromosome stability in all living species. In Eubacteria, the initial stages of the repair process are carried out by the UvrABC excinuclease complex. Despite the wealth of structural data available, some crucial details of the pathway remain elusive. In this study, we present a structural investigation of the Mycobacterium tuberculosis UvrAUvrB complex and of the UvrA dimer, both in complex with damaged DNA. Our analyses yield insights into the DNA binding mode of UvrA, showing an unexplored conformation of Insertion Domains (IDs), underlying the essential role of these domains in DNA coordination. Furthermore, we observe an interplay between the ID and the UvrB Binding Domain (UBD): after the recognition of the damage, the IDs repositions with the concomitant reorganization of UBD, allowing the formation of the complex between UvrA and UvrB. These events are detected along the formation of the uncharacterized UvrAUvrB-DNA and the UvrAUvrB-DNA complexes which we interpret as hierarchical steps initiating the DNA repair cascade in the NER pathway, resulting in the formation of the pre-incision complex.

PubMed: 40210888
DOI: 10.1038/s41467-025-58670-0

実験手法

ELECTRON MICROSCOPY (4.3 Å)