9G7N

Crystal structure of Chromobacterium haemolyticum PE-like toxin, Hmx

This is a non-PDB format compatible entry.

Summary for 9G7N

• Entry DOI: 10.2210/pdb9g7n/pdb
• Descriptor: Exotoxin A, IMIDAZOLE, ACETATE ION, ... (4 entities in total)
• Functional Keywords: mono-adp ribosylating toxin, exotoxin a, bacterial toxin, toxin
• Biological source: Chromobacterium haemolyticum
• Total number of polymer chains: 1
• Total formula weight: 68618.26

Authors

Masuyer, G. (deposition date: 2024-07-22, release date: 2025-01-29, Last modification date: 2025-03-26)

Primary citation

Masuyer, G.,Taverner, A.,MacKay, J.,Lima Marques, A.R.,Wang, Y.,Hunter, T.,Liu, K.,Mrsny, R.J.
Discovery of mono-ADP ribosylating toxins with high structural homology to Pseudomonas exotoxin A.
Commun Biol, 8:413-413, 2025

PubMed Abstract: Mono-ADP-ribosyl transferase (mART) proteins are secreted virulence factors produced by several human pathogens, the founding member being diphtheria toxin (DT). Pseudomonas aeruginosa can also secrete a mART toxin, known as exotoxin A (PE), but with an organization of its three functional domains (receptor, translocation, and enzymatic elements) that is opposite to DT. Two additional PE-like toxins (PLTs) have been identified from Vibrio cholerae and Aeromonas hydrophila, suggesting more PLT family members may exist. Database mining discovered six additional putative homologues, considerably extending this group of PLTs across a wide range of bacterial species. Here, we examine sequence and structural information for these new family members with respect to previously identified PLTs. The X-ray crystal structures of four new homologues show the conservation of critical features responsible for structure and function. This study shows the potential of these newly described toxins for the development of novel drug delivery platforms. Additionally, genomic analysis suggests horizontal gene transfer to account for the wide distribution of PLTs across a range of eubacteria species, highlighting the need to monitor emerging pathogens and their virulence factors.

PubMed: 40069285
DOI: 10.1038/s42003-025-07845-y

Experimental method

X-RAY DIFFRACTION (1.35 Å)