9G7C
Cryo-EM structure of raiA ncRNA from Clostridium reveals a new RNA 3D fold
Summary for 9G7C
| Entry DOI | 10.2210/pdb9g7c/pdb |
| EMDB information | 51119 |
| Descriptor | raiA (224-MER) (1 entity in total) |
| Functional Keywords | ncrna, raia, rna |
| Biological source | Clostridium sp. CAG:138 |
| Total number of polymer chains | 1 |
| Total formula weight | 72829.30 |
| Authors | Badepally, N.G.,Moura, T.R.,Purta, E.,Baulin, E.,Bujnicki, J.M. (deposition date: 2024-07-20, release date: 2024-10-30, Last modification date: 2024-11-13) |
| Primary citation | Badepally, N.G.,de Moura, T.R.,Purta, E.,Baulin, E.F.,Bujnicki, J.M. Cryo-EM Structure of raiA ncRNA From Clostridium Reveals a New RNA 3D Fold. J.Mol.Biol., 436:168833-168833, 2024 Cited by PubMed Abstract: Advancements in genome-wide sequence analysis have led to the discovery of numerous novel bacterial non-coding RNAs (ncRNAs). These ncRNAs have been categorized into various RNA families and classes based on their size, structure, function, and evolutionary relationships. One such ncRNA family, raiA, is notably abundant in the bacterial phyla Firmicutes and Actinobacteria and is remarkably well-conserved across many Gram-positive bacteria. In this study, we integrated cryo-electron microscopy single-particle analysis with computational modeling and biochemical techniques to elucidate the structural characteristics of raiA from Clostridium sp. CAG 138. Our findings reveal the globular 3D fold of raiA, providing valuable structural insights. This analysis paves the way for future investigations into the functional properties of raiA, potentially uncovering new regulatory mechanisms in bacterial ncRNAs. PubMed: 39454748DOI: 10.1016/j.jmb.2024.168833 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.27 Å) |
Structure validation
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