9G7C

Cryo-EM structure of raiA ncRNA from Clostridium reveals a new RNA 3D fold

9G7C の概要

• エントリーDOI: 10.2210/pdb9g7c/pdb
• EMDBエントリー: 51119
• 分子名称: raiA (224-MER) (1 entity in total)
• 機能のキーワード: ncrna, raia, rna
• 由来する生物種: Clostridium sp. CAG:138
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 72829.30

構造登録者

Badepally, N.G.,Moura, T.R.,Purta, E.,Baulin, E.,Bujnicki, J.M. (登録日: 2024-07-20, 公開日: 2024-10-30, 最終更新日: 2024-11-13)

主引用文献

Badepally, N.G.,de Moura, T.R.,Purta, E.,Baulin, E.F.,Bujnicki, J.M.
Cryo-EM Structure of raiA ncRNA From Clostridium Reveals a New RNA 3D Fold.
J.Mol.Biol., 436:168833-168833, 2024

PubMed Abstract: Advancements in genome-wide sequence analysis have led to the discovery of numerous novel bacterial non-coding RNAs (ncRNAs). These ncRNAs have been categorized into various RNA families and classes based on their size, structure, function, and evolutionary relationships. One such ncRNA family, raiA, is notably abundant in the bacterial phyla Firmicutes and Actinobacteria and is remarkably well-conserved across many Gram-positive bacteria. In this study, we integrated cryo-electron microscopy single-particle analysis with computational modeling and biochemical techniques to elucidate the structural characteristics of raiA from Clostridium sp. CAG 138. Our findings reveal the globular 3D fold of raiA, providing valuable structural insights. This analysis paves the way for future investigations into the functional properties of raiA, potentially uncovering new regulatory mechanisms in bacterial ncRNAs.

PubMed: 39454748
DOI: 10.1016/j.jmb.2024.168833

実験手法

ELECTRON MICROSCOPY (3.27 Å)