9G5C

Assembly intermediate of human mitochondrial ribosome small subunit (State B)

9G5C の概要

• エントリーDOI: 10.2210/pdb9g5c/pdb
• EMDBエントリー: 51052 51084
• 分子名称: 28S ribosomal protein S34, mitochondrial, 28S ribosomal protein S7, mitochondrial, 28S ribosomal protein S9, mitochondrial, ... (39 entities in total)
• 機能のキーワード: mitochondrial ribosomal small subunit, assembly intermediate, immature h44, single-particle cryo-em, ribosome
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 32
• 化学式量合計: 1215526.52

構造登録者

Finke, A.F.,Heinrichs, M.,Aibara, S.,Richter-Dennerlein, R.,Hillen, H.S. (登録日: 2024-07-16, 公開日: 2025-04-23, 最終更新日: 2025-04-30)

主引用文献

Heinrichs, M.,Finke, A.F.,Aibara, S.,Krempler, A.,Boshnakovska, A.,Rehling, P.,Hillen, H.S.,Richter-Dennerlein, R.
Coupling of ribosome biogenesis and translation initiation in human mitochondria.
Nat Commun, 16:3641-3641, 2025

PubMed Abstract: Biogenesis of mitoribosomes requires dedicated chaperones, RNA-modifying enzymes, and GTPases, and defects in mitoribosome assembly lead to severe mitochondriopathies in humans. Here, we characterize late-step assembly states of the small mitoribosomal subunit (mtSSU) by combining genetic perturbation and mutagenesis analysis with biochemical and structural approaches. Isolation of native mtSSU biogenesis intermediates via a FLAG-tagged variant of the GTPase MTG3 reveals three distinct assembly states, which show how factors cooperate to mature the 12S rRNA. In addition, we observe four distinct primed initiation mtSSU states with an incompletely matured rRNA, suggesting that biogenesis and translation initiation are not mutually exclusive processes but can occur simultaneously. Together, these results provide insights into mtSSU biogenesis and suggest a functional coupling between ribosome biogenesis and translation initiation in human mitochondria.

PubMed: 40240327
DOI: 10.1038/s41467-025-58827-x

実験手法

ELECTRON MICROSCOPY (3 Å)