9G2U

Endophilin B1 dimer bound to nanodisc center

9G2U の概要

• エントリーDOI: 10.2210/pdb9g2u/pdb
• 関連するPDBエントリー: 9G2R 9G2W
• EMDBエントリー: 50984
• 分子名称: Endophilin-B1 (1 entity in total)
• 機能のキーワード: bar, n-bar, endophilin, membrane, curvature, cardiolipin, msp2n2, nanodisc, peripheral membrane protein, apoptosis
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 81686.49

構造登録者

Thorlacius, A.,Sundborger-Lunna, A. (登録日: 2024-07-11, 公開日: 2024-08-07, 最終更新日: 2025-02-19)

主引用文献

Thorlacius, A.,Rulev, M.,Sundberg, O.,Sundborger-Lunna, A.
Peripheral membrane protein endophilin B1 probes, perturbs and permeabilizes lipid bilayers.
Commun Biol, 8:182-182, 2025

PubMed Abstract: Bin/Amphiphysin/Rvs167 (BAR) domain containing proteins are peripheral membrane proteins that regulate intracellular membrane curvature. BAR protein endophilin B1 plays a key role in multiple cellular processes critical for oncogenesis, including autophagy and apoptosis. Amphipathic regions in endophilin B1 drive membrane association and tubulation through membrane scaffolding. Our understanding of exactly how BAR proteins like endophilin B1 promote highly diverse intracellular membrane remodeling events in the cell is severely limited due to lack of high-resolution structural information. Here we present the highest resolution cryo-EM structure of a BAR protein to date and the first structures of a BAR protein bound to a lipid bicelle. Using neural networks, we can effectively sort particle species of different stoichiometries, revealing the tremendous flexibility of post-membrane binding, pre-polymer BAR dimer organization and membrane deformation. We also show that endophilin B1 efficiently permeabilizes negatively charged liposomes that contain mitochondria-specific lipid cardiolipin and propose a new model for Bax-mediated cell death.

PubMed: 39910321
DOI: 10.1038/s42003-025-07610-1

実験手法

ELECTRON MICROSCOPY (3.45 Å)