9G28

snR30 snoRNP - State 2 - Utp23-Krr1-deltaC3

9G28 の概要

• エントリーDOI: 10.2210/pdb9g28/pdb
• EMDBエントリー: 50968
• 分子名称: RDN18-1, 40S ribosomal protein S14-A, rRNA-processing protein UTP23, ... (11 entities in total)
• 機能のキーワード: 90s, pre-ribosome, snorna, snr30, snornp, ribosome biogenesis, h/aca, 18s rrna, ribosome
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast); 詳細
• タンパク質・核酸の鎖数: 14
• 化学式量合計: 1120412.04

構造登録者

Thoms, M.,Berninghausen, O.,Beckmann, R. (登録日: 2024-07-10, 公開日: 2025-06-18)

主引用文献

Fischer, P.,Thoms, M.,Lau, B.,Denk, T.,Kuvshinova, M.,Berninghausen, O.,Flemming, D.,Hurt, E.,Beckmann, R.
H/ACA snR30 snoRNP guides independent 18S rRNA subdomain formation.
Nat Commun, 16:4720-4720, 2025

PubMed Abstract: Ribosome biogenesis follows a cascade of pre-rRNA folding and processing steps, coordinated with ribosomal protein incorporation. Nucleolar 90S pre-ribosomes are well-described stable intermediates, composed of pre-18S rRNA, ribosomal S-proteins, U3 snoRNA, and ~70 assembly factors. However, how numerous snoRNAs control pre-rRNA modification and folding during early maturation events remains unclear. We identify snR30 (human U17), the only essential H/ACA snoRNA in yeast, which binds with Cbf5-Gar1-Nop10-Nhp2 to a pre-18S rRNA subdomain containing platform helices and ES6 of the 40S central domain. Integration into the 90S is blocked by RNA hybridization with snR30. The snoRNP complex coordinates the recruitment of early assembly factors Krr1-Utp23-Kri1 and ribosomal proteins uS11-uS15, enabling isolated subdomain assembly. Krr1-dependent release of snR30 culminates in integration of the platform into the 90S. Our study reveals the essential role of snR30 in chaperoning central domain formation as a discrete assembly unit externalized from the pre-ribosomal core.

PubMed: 40399280
DOI: 10.1038/s41467-025-59656-8

実験手法

ELECTRON MICROSCOPY (3.18 Å)