9G0X
auxin transporter PIN8 as asymmetric dimer (inward/outward) with 4-CPA bound in the inward vestibule prebinding state
This is a non-PDB format compatible entry.
Summary for 9G0X
| Entry DOI | 10.2210/pdb9g0x/pdb |
| EMDB information | 50951 |
| Descriptor | Auxin efflux carrier component 8, 2-(4-chloranylphenoxy)ethanoic acid, 1,2-DILINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE, ... (4 entities in total) |
| Functional Keywords | auxin transport, aec family, bart superfamily, membrane protein, transport protein |
| Biological source | Arabidopsis thaliana (thale cress) |
| Total number of polymer chains | 2 |
| Total formula weight | 84832.83 |
| Authors | Ung, K.L.,Stokes, D.L.,Pedersen, B.P. (deposition date: 2024-07-09, release date: 2025-04-09, Last modification date: 2025-04-16) |
| Primary citation | Schulz, L.,Ung, K.L.,Koutnik-Abele, S.,Stokes, D.L.,Pedersen, B.P.,Hammes, U.Z. Transport of herbicides by PIN-FORMED auxin transporters. Biorxiv, 2024 Cited by PubMed Abstract: Auxins are a group of phytohormones that control plant growth and development . Their crucial role in plant physiology has inspired development of potent synthetic auxins that can be used as herbicides . Phenoxyacetic acid derivatives are a widely used group of auxin herbicides in agriculture and research. Despite their prevalence, the identity of the transporters required for distribution of these herbicides in plants is both poorly understood and the subject of controversial debate . Here we show that PIN-FORMED auxin transporters transport a range of phenoxyacetic acid herbicides across the membrane and we characterize the molecular determinants of this process using a variety of different substrates as well as protein mutagenesis to control substrate specificity. Finally, we present Cryo-EM structures of PIN8 with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-chlorophenoxyacetic acid (4-CPA) bound. These structures represent five key states from the transport cycle, allowing us to describe conformational changes associated with substrate binding and transport across the membrane. Overall, our results reveal that phenoxyacetic acid herbicides use the same export machinery as endogenous auxins and exemplify how transporter binding sites undergo transformations that dictate substrate specificity. These results enable development of novel synthetic auxins and for guiding precision breeding of herbicide resistant crop plants. PubMed: 39257797DOI: 10.1101/2024.08.29.610046 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (3.38 Å) |
Structure validation
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