9G0X

auxin transporter PIN8 as asymmetric dimer (inward/outward) with 4-CPA bound in the inward vestibule prebinding state

これはPDB形式変換不可エントリーです。

9G0X の概要

• エントリーDOI: 10.2210/pdb9g0x/pdb
• EMDBエントリー: 50951
• 分子名称: Auxin efflux carrier component 8, 2-(4-chloranylphenoxy)ethanoic acid, 1,2-DILINOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE, ... (4 entities in total)
• 機能のキーワード: auxin transport, aec family, bart superfamily, membrane protein, transport protein
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 84832.83

構造登録者

Ung, K.L.,Stokes, D.L.,Pedersen, B.P. (登録日: 2024-07-09, 公開日: 2025-04-09, 最終更新日: 2025-04-16)

主引用文献

Schulz, L.,Ung, K.L.,Koutnik-Abele, S.,Stokes, D.L.,Pedersen, B.P.,Hammes, U.Z.
Transport of herbicides by PIN-FORMED auxin transporters.
Biorxiv, 2024

PubMed Abstract: Auxins are a group of phytohormones that control plant growth and development . Their crucial role in plant physiology has inspired development of potent synthetic auxins that can be used as herbicides . Phenoxyacetic acid derivatives are a widely used group of auxin herbicides in agriculture and research. Despite their prevalence, the identity of the transporters required for distribution of these herbicides in plants is both poorly understood and the subject of controversial debate . Here we show that PIN-FORMED auxin transporters transport a range of phenoxyacetic acid herbicides across the membrane and we characterize the molecular determinants of this process using a variety of different substrates as well as protein mutagenesis to control substrate specificity. Finally, we present Cryo-EM structures of PIN8 with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-chlorophenoxyacetic acid (4-CPA) bound. These structures represent five key states from the transport cycle, allowing us to describe conformational changes associated with substrate binding and transport across the membrane. Overall, our results reveal that phenoxyacetic acid herbicides use the same export machinery as endogenous auxins and exemplify how transporter binding sites undergo transformations that dictate substrate specificity. These results enable development of novel synthetic auxins and for guiding precision breeding of herbicide resistant crop plants.

PubMed: 39257797
DOI: 10.1101/2024.08.29.610046

実験手法

ELECTRON MICROSCOPY (3.38 Å)