9G0P

Xenopus laevis undecorated microtubule - 14 protofilament, 3-start helix

9G0P の概要

• エントリーDOI: 10.2210/pdb9g0p/pdb
• 関連するPDBエントリー: 9FVJ 9G0O 9G0Q 9G0R 9G0S 9G0T
• EMDBエントリー: 50942
• 分子名称: Tubulin beta-4 chain, Tubulin alpha chain, GUANOSINE-5'-DIPHOSPHATE, ... (5 entities in total)
• 機能のキーワード: microtubule tubulin cytoskeleton filament, protein fibril
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 605830.37

構造登録者

Troman, L.A.,Moores, C.A. (登録日: 2024-07-08, 公開日: 2025-01-15, 最終更新日: 2025-02-12)

主引用文献

Troman, L.,de Gaulejac, E.,Biswas, A.,Stiens, J.,Kuropka, B.,Moores, C.A.,Reber, S.
Mechanistic basis of temperature adaptation in microtubule dynamics across frog species.
Curr.Biol., 35:612-628.e6, 2025

PubMed Abstract: Cellular processes are remarkably effective across diverse temperature ranges, even with highly conserved proteins. In the context of the microtubule cytoskeleton, which is critically involved in a wide range of cellular activities, this is particularly striking, as tubulin is one of the most conserved proteins while microtubule dynamic instability is highly temperature sensitive. Here, we leverage the diversity of natural tubulin variants from three closely related frog species that live at different temperatures. We determine the microtubule structure across all three species at between 3.0 and 3.6 Å resolution by cryo-electron microscopy and find small differences at the β-tubulin lateral interactions. Using in vitro reconstitution assays and quantitative biochemistry, we show that tubulin's free energy scales inversely with temperature. The observed weakening of lateral contacts and the low apparent activation energy for tubulin incorporation provide an explanation for the overall stability and higher growth rates of microtubules in cold-adapted frog species. This study thus broadens our conceptual framework for understanding microtubule dynamics and provides insights into how conserved cellular processes are tailored to different ecological niches.

PubMed: 39798564
DOI: 10.1016/j.cub.2024.12.022
主引用文献が同じPDBエントリー

実験手法

ELECTRON MICROSCOPY (3 Å)