9G0F

CryoEM structure of PmcTnsC-dsDNA-AMPPNP

Summary for 9G0F

• Entry DOI: 10.2210/pdb9g0f/pdb
• EMDB information: 50932
• Descriptor: AAA+ ATPase domain-containing protein, DNA, PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER, ... (5 entities in total)
• Functional Keywords: aaa+ atpase, crispr, tn7-like transposon, cast, transposase, dna binding protein
• Biological source: Peltigera membranacea; More
• Total number of polymer chains: 9
• Total formula weight: 320069.23

Authors

Finocchio, G.,Chanez, C.,Querques, I.,Speichert, K.J.,Jinek, M. (deposition date: 2024-07-08, release date: 2025-04-02, Last modification date: 2025-07-09)

Primary citation

Finocchio, G.,Querques, I.,Chanez, C.,Speichert, K.J.,Jinek, M.
Structural basis of TnsC oligomerization and transposase recruitment in type I-B CRISPR-associated transposons.
Nucleic Acids Res., 53:-, 2025

PubMed Abstract: CRISPR-associated transposon (CAST) systems employ CRISPR-Cas systems as RNA-directed targeting modules for site-specific transposon DNA insertion. Among them, type I CASTs rely on the coordinated action of the guide RNA-bound Cascade complex and the transposon proteins TniQ, TnsC, and TnsAB. The interaction between the transposase TnsAB and the ATPase TnsC is crucial for transposition activity, yet the underlying molecular details have remained elusive. Here, we investigate the type I-B CAST system from Peltigera membranacea cyanobiont. Cryo-electron microscopic structures of TnsC and its complex with the C-terminal region of TnsAB reveal that TnsC forms a heptameric ring that recruits TnsAB by interacting with its C-terminal tail. In vitro binding assays indicate that TnsAB exclusively interacts with the TnsC heptamer without inducing its disassembly, in contrast to type V-K CAST systems. Mutational analysis of key structural features corroborates the significance of TnsC multimerization and TnsB interaction for transposon activity in vivo. Altogether, these findings offer detailed structural and functional insights into the molecular mechanism of type I-B CAST, with the aim of facilitating their development as genome engineering tools.

PubMed: 40103227
DOI: 10.1093/nar/gkaf149

Experimental method

ELECTRON MICROSCOPY (3.7 Å)