9FTY

Serial microseconds crystallography at ID29 using fixed-target (small foils): Proteinase K with 20 um spacing between X-ray pulses

9FTY の概要

• エントリーDOI: 10.2210/pdb9fty/pdb
• 分子名称: Proteinase K, CALCIUM ION, NITRATE ION, ... (4 entities in total)
• 機能のキーワード: serial crystallography, serial microsecond crystallography, room temperature, id29, esrf-ebs, hydrolase
• 由来する生物種: Parengyodontium album
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 29100.95

構造登録者

Orlans, J.,Rose, S.L.,Basu, S.,de Sanctis, D. (登録日: 2024-06-25, 公開日: 2025-01-22)

主引用文献

Orlans, J.,Rose, S.L.,Ferguson, G.,Oscarsson, M.,Homs Puron, A.,Beteva, A.,Debionne, S.,Theveneau, P.,Coquelle, N.,Kieffer, J.,Busca, P.,Sinoir, J.,Armijo, V.,Lopez Marrero, M.,Felisaz, F.,Papp, G.,Gonzalez, H.,Caserotto, H.,Dobias, F.,Gigmes, J.,Lebon, G.,Basu, S.,de Sanctis, D.
Advancing macromolecular structure determination with microsecond X-ray pulses at a 4th generation synchrotron.
Commun Chem, 8:6-6, 2025

PubMed Abstract: Serial macromolecular crystallography has become a powerful method to reveal room temperature structures of biological macromolecules and perform time-resolved studies. ID29, a flagship beamline of the ESRF 4th generation synchrotron, is the first synchrotron beamline in the world capable of delivering high brilliance microsecond X-ray pulses at high repetition rate for the structure determination of biological macromolecules at room temperature. The cardinal combination of microsecond exposure times, innovative beam characteristics and adaptable sample environment provides high quality complete data, even from an exceptionally small amount of crystalline material, enabling what we collectively term serial microsecond crystallography (SµX). After validating the use of different sample delivery methods with various model systems, we applied SµX to an integral membrane receptor, where only a few thousands diffraction images were sufficient to obtain a fully interpretable electron density map for the antagonist istradefylline-bound A receptor conformation, providing access to the antagonist binding mode. SµX, as demonstrated at ID29, will quickly find its broad applicability at upcoming 4th generation synchrotron sources worldwide and opens a new frontier in time-resolved SµX.

PubMed: 39775172
DOI: 10.1038/s42004-024-01404-y

実験手法

X-RAY DIFFRACTION (2 Å)