9FPM

Crystal structure of the Tin2-fold effector protein Tue1 from Thecaphora thlaspeos

9FPM の概要

• エントリーDOI: 10.2210/pdb9fpm/pdb
• 分子名称: Tue1 (2 entities in total)
• 機能のキーワード: nuclear-localization sequence, thecaphora thlaspeos, jaz, jasmonate regulation, nuclear protein
• 由来する生物種: Thecaphora thlaspeos
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 31501.90

構造登録者

Mendoza-Rojas, G.,Altegoer, F. (登録日: 2024-06-13, 公開日: 2025-06-25, 最終更新日: 2025-12-17)

主引用文献

Gul, S.,Mendoza-Rojas, G.,Heinen, M.,Hessler, N.,Galle, S.,Zeier, J.,Smits, S.H.J.,Altegoer, F.,Gohre, V.
Jasmonic acid signalling is targeted by a smut fungal Tin2-fold effector.
J.Exp.Bot., 76:7051-7066, 2025

PubMed Abstract: Jasmonate signalling integrates environmental cues with plant growth and development. Owing to its role in balancing defence responses against pathogens, it is a target of effector proteins from various pathogens. Here, we characterized the fungal effector protein Tue1 from the Brassicaceae smut fungus Thecaphora thlaspeos, which naturally infects Arabis hirsuta but can also colonize Arabidopsis thaliana. In planta, Tue1 hijacks the plant importin-α-dependent nuclear transporter to reach the plant nucleus. We demonstrate that Tue1 interacts with jasmonate ZIM-domain 10 (JAZ10) proteins of both A. thaliana and A. hirsuta. Structure-guided analysis of Tue1 suggests that it binds the Jas motif of JAZ10, indicating a role in stabilization or binding competition with proteins such as MYC3 and COI1. This is further supported by reduced jasmonate concentrations upon tue1 overexpression in A. thaliana and differential regulation of a subset of jasmonate-responsive genes during T. thlaspeos infection of A. hirsuta. Tue1 is part of the Tin2-fold effector family recently described in the corn smut Ustilago maydis. Our study demonstrates that this effector family is expanded across smut fungal species, although further studies are needed to reveal whether targeting JAZ repressors is a conserved mechanism or specifically acquired as an adaptation to the perennial host.

PubMed: 40891345
DOI: 10.1093/jxb/eraf381

実験手法

X-RAY DIFFRACTION (1.7 Å)