9FPE
Wild type Purine Nucleoside Phosphorylase from E.coli in complex with N2,3-etheno-2-aminopurine
This is a non-PDB format compatible entry.
Summary for 9FPE
Entry DOI | 10.2210/pdb9fpe/pdb |
Descriptor | Purine nucleoside phosphorylase DeoD-type, GLYCEROL, N,2,3-etheno-2-aminopurine, ... (6 entities in total) |
Functional Keywords | fluorescent ligand, transferase |
Biological source | Escherichia coli K-12 |
Total number of polymer chains | 3 |
Total formula weight | 79272.67 |
Authors | Narczyk, M.,Krystian, M.,Stachelska-Wierzchowska, A.,Wierzchowski, J. (deposition date: 2024-06-13, release date: 2024-10-30) |
Primary citation | Stachelska-Wierzchowska, A.,Narczyk, M.,Wierzchowski, J.,Bzowska, A.,Wielgus-Kutrowska, B. Interaction of Tri-Cyclic Nucleobase Analogs with Enzymes of Purine Metabolism: Xanthine Oxidase and Purine Nucleoside Phosphorylase. Int J Mol Sci, 25:-, 2024 Cited by PubMed Abstract: Fluorescent markers play important roles in spectroscopic and microscopic research techniques and are broadly used in basic and applied sciences. We have obtained markers with fluorescent properties, two etheno derivatives of 2-aminopurine, as follows: 1,N-etheno-2-aminopurine (1,N-ε2APu, ) and N,3-etheno-2-aminopurine (N,3-ε2APu, ). In the present paper, we investigate their interaction with two key enzymes of purine metabolism, purine nucleoside phosphorylase (PNP), and xanthine oxidase (XO), using diffraction of X-rays on protein crystals, isothermal titration calorimetry, and fluorescence spectroscopy. Crystals were obtained and structures were solved for WT PNP and D204N-PNP mutant in a complex with N,3-ε2APu (). In the case of WT PNP-1,N-ε2APu () complex, the electron density corresponding to the ligand could not be identified in the active site. Small electron density bobbles may indicate that the ligand binds to the active site of a small number of molecules. On the basis of spectroscopic studies in solution, we found that, in contrast to PNP, 1,N-ε2APu () is the ligand with better affinity to XO. Enzymatic oxidation of () leads to a marked increase in fluorescence near 400 nm. Hence, we have developed a new method to determine XO activity in biological material, particularly suitable for milk analysis. PubMed: 39408755DOI: 10.3390/ijms251910426 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.35 Å) |
Structure validation
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