9FO3

Structure of a gp140 SpyTag-SpyCatcher mi3 nanoparticle including mi3 density only.

9FO3 の概要

• エントリーDOI: 10.2210/pdb9fo3/pdb
• EMDBエントリー: 50615
• 分子名称: CAP255 gp140 SpyTag-SpyCatcher mi3,2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase,2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase (1 entity in total)
• 機能のキーワード: vaccine, hiv-1, mi3, nanoparticle, vlp, immune system
• 由来する生物種: Thermotoga maritima; 詳細
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 2033686.62

構造登録者

Woodward, J.D.,Malebo, K.,Chapman, R. (登録日: 2024-06-11, 公開日: 2024-10-09, 最終更新日: 2024-11-13)

主引用文献

Malebo, K.,Woodward, J.,Ximba, P.,Mkhize, Q.,Cingo, S.,Moyo-Gwete, T.,Moore, P.L.,Williamson, A.L.,Chapman, R.
Development of a Two-Component Nanoparticle Vaccine Displaying an HIV-1 Envelope Glycoprotein that Elicits Tier 2 Neutralising Antibodies.
Vaccines (Basel), 12:-, 2024

PubMed Abstract: Despite treatment and other interventions, an effective prophylactic HIV vaccine is still an essential goal in the control of HIV. Inducing robust and long-lasting antibody responses is one of the main targets of an HIV vaccine. The delivery of HIV envelope glycoproteins (Env) using nanoparticle (NP) platforms has been shown to elicit better immunogenicity than soluble HIV Env. In this paper, we describe the development of a nanoparticle-based vaccine decorated with HIV Env using the SpyCatcher/SpyTag system. The Env utilised in this study, CAP255, was derived from a transmitted founder virus isolated from a patient who developed broadly neutralising antibodies. Negative stain and cryo-electron microscopy analyses confirmed the assembly and stability of the mi3 into uniform icosahedral NPs surrounded by regularly spaced CAP255 gp140 Env trimers. A three-dimensional reconstruction of CAP255 gp140 SpyTag-SpyCatcher mi3 clearly showed Env trimers projecting from the centre of each of the pentagonal dodecahedral faces of the NP. To our knowledge, this is the first study to report the formation of SpyCatcher pentamers on the dodecahedral faces of mi3 NPs. To investigate the immunogenicity, rabbits were primed with two doses of DNA vaccines expressing the CAP255 gp150 and a mosaic subtype C Gag and boosted with three doses of the NP-developed autologous Tier 2 CAP255 neutralising antibodies (Nabs) and low levels of heterologous CAP256SU NAbs.

PubMed: 39340093
DOI: 10.3390/vaccines12091063

実験手法

ELECTRON MICROSCOPY (5.3 Å)