9FLY

CryoEM structure of the base (4151-5009) in the grappling hook protein A (GhpA) in the bacterium Aureispira sp. CCB-QB1

9FLY の概要

• エントリーDOI: 10.2210/pdb9fly/pdb
• EMDBエントリー: 50543
• 分子名称: The grappling hook protein A in the bacterium Aureispira sp. CCB-QB1 (1 entity in total)
• 機能のキーワード: ixotrophy, type 9 secretion system, cryoem, surface protein, predation, cell adhesion
• 由来する生物種: Aureispira sp. CCB-QB1
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 4270800.63

構造登録者

Lien, Y.-W.,Amendola, D.,Lee, K.S.,Bartlau, N.,Xu, J.,Furusawa, G.,Polz, M.F.,Stocker, R.,Weiss, G.L.,Pilhofer, M. (登録日: 2024-06-05, 公開日: 2024-10-16, 最終更新日: 2024-10-30)

主引用文献

Lien, Y.W.,Amendola, D.,Lee, K.S.,Bartlau, N.,Xu, J.,Furusawa, G.,Polz, M.F.,Stocker, R.,Weiss, G.L.,Pilhofer, M.
Mechanism of bacterial predation via ixotrophy.
Science, 386:eadp0614-eadp0614, 2024

PubMed Abstract: Ixotrophy is a contact-dependent predatory strategy of filamentous bacteria in aquatic environments for which the molecular mechanism remains unknown. We show that predator-prey contact can be established by gliding motility or extracellular assemblages we call "grappling hooks." Cryo-electron microscopy identified the grappling hooks as heptamers of a type IX secretion system substrate. After close predator-prey contact is established, cryo-electron tomography and functional assays showed that puncturing by a type VI secretion system mediated killing. Single-cell analyses with stable isotope-labeled prey revealed that prey components are taken up by the attacker. Depending on nutrient availability, insertion sequence elements toggle the activity of ixotrophy. A marine metagenomic time series shows coupled dynamics of ixotrophic bacteria and prey. We found that the mechanism of ixotrophy involves multiple cellular machineries, is conserved, and may shape microbial populations in the environment.

PubMed: 39418385
DOI: 10.1126/science.adp0614

実験手法

ELECTRON MICROSCOPY (3.7 Å)