9FKA

Cryo-EM structure of the reduced cytochrome bd oxidase from M. tuberculosis

9FKA の概要

• エントリーDOI: 10.2210/pdb9fka/pdb
• EMDBエントリー: 50520
• 分子名称: Probable integral membrane cytochrome D ubiquinol oxidase (Subunit I) CydA (Cytochrome BD-I oxidase subunit I), Probable integral membrane cytochrome D ubiquinol oxidase (Subunit II) CydB (Cytochrome BD-I oxidase subunit II), PHOSPHATIDYLETHANOLAMINE, ... (8 entities in total)
• 機能のキーワード: ubiquinone, demethylmenaquinone, cryo-em, respiration, substrate specificity, disulfide regulation, oxidoreductase
• 由来する生物種: Mycobacterium tuberculosis H37Rv; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 97604.46

構造登録者

Kayastha, K.,Bruenle, S. (登録日: 2024-06-03, 公開日: 2025-02-12)

主引用文献

van der Velden, T.T.,Kayastha, K.,Waterham, C.Y.J.,Brunle, S.,Jeuken, L.J.C.
Menaquinone-specific turnover by Mycobacterium tuberculosis cytochrome bd is redox regulated by the Q-loop disulfide bond.
J.Biol.Chem., 301:108094-108094, 2024

PubMed Abstract: Cytochrome bd from Mycobacterium tuberculosis (Mtbd) is a menaquinol oxidase that has gained interest as an antibiotic target because of its importance in survival under infectious conditions. Mtbd contains a characteristic disulfide bond that has been hypothesized to allow for Mtbd activity regulation at the enzymatic level, possibly helping M. tuberculosis to rapidly adapt to the hostile environment of the phagosome. Here, the role of the disulfide bond and quinone specificity have been determined by reconstitution of a minimal respiratory chain and the single-particle cryo-EM structure in the disulfide-reduced form. Mtbd was shown to be specific for menaquinone, while regulation by reduction of the Q-loop disulfide bond decreased oxidase activity up to 90%. Structural analysis shows that a salt bridge unique to Mtbd keeps the Q-loop partially structured in its disulfide-reduced form, which could facilitate the rapid activation of Mtbd upon exposure to reactive oxygen species. We signify Mtbd as the first redox sensory terminal oxidase and propose that this helps M. tuberculosis in the defense against reactive oxygen species encountered during infection.

PubMed: 39706268
DOI: 10.1016/j.jbc.2024.108094

実験手法

ELECTRON MICROSCOPY (2.96 Å)