9FI8

SSU(head) structure derived from the SSU sample of the mitoribosome from T. gondii.

これはPDB形式変換不可エントリーです。

9FI8 の概要

• エントリーDOI: 10.2210/pdb9fi8/pdb
• 関連するPDBエントリー: 9FIA 9G6K
• EMDBエントリー: 50448
• 分子名称: Small ribosomal subunit protein mS29,mS29(DAP3), 30S ribosomal protein S5, putative, mS155, ... (28 entities in total)
• 機能のキーワード: complex, translation, rrna, ribosome
• 由来する生物種: Toxoplasma gondii; 詳細
• タンパク質・核酸の鎖数: 28
• 化学式量合計: 656683.38

構造登録者

Rocha, R.E.O.,Barua, S.,Boissier, F.,Nguyen, T.T.,Hashem, Y. (登録日: 2024-05-28, 公開日: 2024-12-11, 最終更新日: 2025-01-01)

主引用文献

Wang, C.,Kassem, S.,Rocha, R.E.O.,Sun, P.,Nguyen, T.T.,Kloehn, J.,Liu, X.,Brusini, L.,Bonavoglia, A.,Barua, S.,Boissier, F.,Lucia Del Cistia, M.,Peng, H.,Tang, X.,Xie, F.,Wang, Z.,Vadas, O.,Suo, X.,Hashem, Y.,Soldati-Favre, D.,Jia, Y.
Apicomplexan mitoribosome from highly fragmented rRNAs to a functional machine.
Nat Commun, 15:10689-10689, 2024

PubMed Abstract: The phylum Apicomplexa comprises eukaryotic parasites that cause fatal diseases affecting millions of people and animals worldwide. Their mitochondrial genomes have been significantly reduced, leaving only three protein-coding genes and highly fragmented mitoribosomal rRNAs, raising challenging questions about mitoribosome composition, assembly and structure. Our study reveals how Toxoplasma gondii assembles over 40 mt-rRNA fragments using exclusively nuclear-encoded mitoribosomal proteins and three lineage-specific families of RNA-binding proteins. Among these are four proteins from the Apetala2/Ethylene Response Factor (AP2/ERF) family, originally known as transcription factors in plants and Apicomplexa, now repurposed as essential mitoribosome components. Cryo-EM analysis of the mitoribosome structure demonstrates how these AP2 proteins function as RNA binders to maintain mitoribosome integrity. The mitoribosome is also decorated with members of lineage-specific RNA-binding proteins belonging to RAP (RNA-binding domain abundant in Apicomplexa) proteins and HPR (heptatricopeptide repeat) families, highlighting the unique adaptations of these parasites. Solving the molecular puzzle of apicomplexan mitoribosome could inform the development of therapeutic strategies targeting organellar translation.

PubMed: 39690155
DOI: 10.1038/s41467-024-55033-z

実験手法

ELECTRON MICROSCOPY (3.6 Å)