9FEJ

Structure of the RNA-dependent RNA polymerase P2 from the bacteriophage Phi8

9FEJ の概要

• エントリーDOI: 10.2210/pdb9fej/pdb
• 分子名称: RNA-directed RNA polymerase, CALCIUM ION (2 entities in total)
• 機能のキーワード: polymerase, viral protein
• 由来する生物種: Pseudomonas phage phi8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 71752.96

構造登録者

Latimer-Smith, M.,Salgado, P.S.,Forsyth, I.,Makeyev, E.,Poranen, M.,Stuart, D.I.,Grimes, J.M.,El Omari, K. (登録日: 2024-05-20, 公開日: 2024-10-16, 最終更新日: 2024-10-30)

主引用文献

Latimer-Smith, M.,Salgado, P.S.,Forsyth, I.,Makeyev, E.,Poranen, M.M.,Stuart, D.I.,Grimes, J.M.,El Omari, K.
Structure of the RNA-dependent RNA polymerase P2 from the cystovirus phi 8.
Sci Rep, 14:23540-23540, 2024

PubMed Abstract: The replication of RNA viruses relies on the activity of RNA-dependent RNA polymerases (RdRps). Despite large variations in their genomic sequences, viral RdRps share a common architecture generally known as a closed right hand. The P2 polymerase of cystovirus φ6 is currently among the best characterized viral RdRps. This polymerase is responsible for carrying out both replication and transcription of the viral double-stranded RNA genome using de novo initiation. Despite the extensive biochemical and structural studies conducted on φ6 P2, further structural information on other cystoviral RdRps is crucial to elucidate the structural and functional diversity of viral RdRps. Here, we have determined the atomic X-ray structure of the RdRp P2 from the φ6-related cystovirus φ8 at 3Å resolution. This structure completes the existing set of structural information on the φ8 polymerase complex and sheds light on the difference and similarities with related cystoviral RdRps.

PubMed: 39384884
DOI: 10.1038/s41598-024-75213-7

実験手法

X-RAY DIFFRACTION (3 Å)