9FBW の概要
| エントリーDOI | 10.2210/pdb9fbw/pdb |
| EMDBエントリー | 50297 |
| 分子名称 | Histone H3, RuvB-like protein 1, RuvB-like protein 2, ... (16 entities in total) |
| 機能のキーワード | chromatin remodelling complex, hexasome, dna binding protein |
| 由来する生物種 | Saccharomyces cerevisiae S288C 詳細 |
| タンパク質・核酸の鎖数 | 18 |
| 化学式量合計 | 809142.60 |
| 構造登録者 | |
| 主引用文献 | Jalal, A.S.B.,Girvan, P.,Chua, E.Y.D.,Liu, L.,Wang, S.,McCormack, E.A.,Skehan, M.T.,Knight, C.L.,Rueda, D.S.,Wigley, D.B. Stabilization of the hexasome intermediate during histone exchange by yeast SWR1 complex. Mol.Cell, 84:3871-, 2024 Cited by PubMed Abstract: The yeast SWR1 complex catalyzes the exchange of histone H2A/H2B dimers in nucleosomes with Htz1/H2B dimers. We use cryoelectron microscopy to determine the structure of an enzyme-bound hexasome intermediate in the reaction pathway of histone exchange, in which an H2A/H2B dimer has been extracted from a nucleosome prior to the insertion of a dimer comprising Htz1/H2B. The structure reveals a key role for the Swc5 subunit in stabilizing the unwrapping of DNA from the histone core of the hexasome. By engineering a crosslink between an Htz1/H2B dimer and its chaperone protein Chz1, we show that this blocks histone exchange by SWR1 but allows the incoming chaperone-dimer complex to insert into the hexasome. We use this reagent to trap an SWR1/hexasome complex with an incoming Htz1/H2B dimer that shows how the reaction progresses to the next step. Taken together the structures reveal insights into the mechanism of histone exchange by SWR1 complex. PubMed: 39226902DOI: 10.1016/j.molcel.2024.08.015 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (4.4 Å) |
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