9F6A

EVA71 E096A native particle

9F6A の概要

• エントリーDOI: 10.2210/pdb9f6a/pdb
• EMDBエントリー: 50221
• 分子名称: VP0, VP3, VP1, ... (4 entities in total)
• 機能のキーワード: native provirion intermediate, virus
• 由来する生物種: Enterovirus A71; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 94816.86

構造登録者

Kingston, N.J.,Stonehouse, N.J.,Rowlands, D.J.,Hogle, J.M.,Filman, D.J.,Snowden, J.S.S. (登録日: 2024-04-30, 公開日: 2024-09-04, 最終更新日: 2024-10-02)

主引用文献

Kingston, N.J.,Snowden, J.S.,Grehan, K.,Hall, P.K.,Hietanen, E.V.,Passchier, T.C.,Polyak, S.J.,Filman, D.J.,Hogle, J.M.,Rowlands, D.J.,Stonehouse, N.J.
Mechanism of enterovirus VP0 maturation cleavage based on the structure of a stabilised assembly intermediate.
Plos Pathog., 20:e1012511-e1012511, 2024

PubMed Abstract: Molecular details of genome packaging are little understood for the majority of viruses. In enteroviruses (EVs), cleavage of the structural protein VP0 into VP4 and VP2 is initiated by the incorporation of RNA into the assembling virion and is essential for infectivity. We have applied a combination of bioinformatic, molecular and structural approaches to generate the first high-resolution structure of an intermediate in the assembly pathway, termed a provirion, which contains RNA and intact VP0. We have demonstrated an essential role of VP0 E096 in VP0 cleavage independent of RNA encapsidation and generated a new model of capsid maturation, supported by bioinformatic analysis. This provides a molecular basis for RNA-dependence, where RNA induces conformational changes required for VP0 maturation, but that RNA packaging itself is not sufficient to induce maturation. These data have implications for understanding production of infectious virions and potential relevance for future vaccine and antiviral drug design.

PubMed: 39298524
DOI: 10.1371/journal.ppat.1012511

実験手法

ELECTRON MICROSCOPY (2.7 Å)