9F0R

VIM-2 in complex with GKV65 (5g) - dynamically chiral phosphonic acid-type metallo-beta-lactamase inhibitors

これはPDB形式変換不可エントリーです。

9F0R の概要

• エントリーDOI: 10.2210/pdb9f0r/pdb
• 分子名称: Metallo-beta-lactamase type 2, FORMIC ACID, [(~{R})-(2-hydroxyphenyl)-(4,5,6,7-tetrahydro-2-benzothiophen-1-ylcarbonylamino)methyl]phosphonic acid, ... (6 entities in total)
• 機能のキーワード: beta-lactamase, metallo-beta-lactamase, inhibitor, antibiotic
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 56038.18

構造登録者

Bosman, R.,Prester, A.,Bartels, K.,Gulyas, K.V.,Erdelyi, M.,Schulz, E.C. (登録日: 2024-04-17, 公開日: 2025-04-30, 最終更新日: 2025-05-07)

主引用文献

Gulyas, K.V.,Zhou, L.,Salamonsen, D.,Prester, A.,Bartels, K.,Bosman, R.,Haffke, P.,Li, J.,Tamasi, V.,Deufel, F.,Thoma, J.,Andersson Rasmussen, A.,Csala, M.,Schroder Leiros, H.K.,Xu, Z.,Widersten, M.,Rohde, H.,Schulz, E.C.,Zhu, W.,Erdelyi, M.
Dynamically chiral phosphonic acid-type metallo-beta-lactamase inhibitors.
Commun Chem, 8:119-119, 2025

PubMed Abstract: Antibiotic resistance is a growing global health threat that risks the lives of millions. Among the resistance mechanisms, that mediated by metallo-β-lactamases is of particular concern as these bacterial enzymes dismantle most β-lactam antibiotics, which are our widest applied and cheapest to produce antibiotic agents. So far, no clinically applicable metallo-β-lactamase inhibitors are available. Aiming to adapt to structural variations, we introduce the inhibitor concept: dynamically chiral phosphonic acids. We demonstrate that they are straightforward to synthesize, penetrate bacterial membranes, inhibit the metallo-β-lactamase enzymes NDM-1, VIM-2 and GIM-1, and are non-toxic to human cells. Mimicking the transition state of β-lactam hydrolysis, they target the Zn ions of the metallo-β-lactamase active site. As a unique feature, both of their stereoisomers bind metallo-β-lactamases, which provides them unparalleled adaptability to the structural diversity of these enzymes, and may allow them to hamper bacteria's ability for resistance development.

PubMed: 40253435
DOI: 10.1038/s42004-025-01510-5

実験手法

X-RAY DIFFRACTION (1.3 Å)