9EZY

Vibrio cholerae DdmD-DdmE holo complex

9EZY の概要

• エントリーDOI: 10.2210/pdb9ezy/pdb
• EMDBエントリー: 50091
• 分子名称: Helicase/UvrB N-terminal domain-containing protein, Vibrio cholerae DdmE, 14 nucleotide DNA guide with terminal 5' phosphate, ... (6 entities in total)
• 機能のキーワード: helicase, nuclease, complex, effector, argonaute, immune system
• 由来する生物種: Vibrio cholerae; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 261134.11

構造登録者

Loeff, L.,Jinek, M. (登録日: 2024-04-14, 公開日: 2024-09-04)

主引用文献

Loeff, L.,Adams, D.W.,Chanez, C.,Stutzmann, S.,Righi, L.,Blokesch, M.,Jinek, M.
Molecular mechanism of plasmid elimination by the DdmDE defense system.
Science, 385:188-194, 2024

PubMed Abstract: Seventh-pandemic strains contain two pathogenicity islands that encode the DNA defense modules DdmABC and DdmDE. In this study, we used cryogenic electron microscopy to determine the mechanistic basis for plasmid defense by DdmDE. The helicase-nuclease DdmD adopts an autoinhibited dimeric architecture. The prokaryotic Argonaute protein DdmE uses a DNA guide to target plasmid DNA. The structure of the DdmDE complex, validated by in vivo mutational studies, shows that DNA binding by DdmE triggers disassembly of the DdmD dimer and loading of monomeric DdmD onto the nontarget DNA strand. In vitro studies indicate that DdmD translocates in the 5'-to-3' direction, while partially degrading the plasmid DNA. These findings provide critical insights into the mechanism of DdmDE systems in plasmid elimination.

PubMed: 38870273
DOI: 10.1126/science.adq0534

実験手法

ELECTRON MICROSCOPY (2.56 Å)