9EZ0

Poliovirus type 1 (strain Mahoney) expanded conformation stabilised virus-like particle (PV1 SC6b) from a yeast expression system.

9EZ0 の概要

• エントリーDOI: 10.2210/pdb9ez0/pdb
• EMDBエントリー: 50066
• 分子名称: Capsid protein VP1, Capsid protein VP0, Capsid protein VP3 (3 entities in total)
• 機能のキーワード: capsid protein, virus-like particle, vaccine, virus like particle
• 由来する生物種: Human poliovirus 1 Mahoney; 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 97463.95

構造登録者

Bahar, M.W.,Sherry, L.,Stonehouse, N.J.,Rowlands, D.J.,Fry, E.E.,Stuart, D.I. (登録日: 2024-04-10, 公開日: 2025-01-29)

主引用文献

Sherry, L.,Bahar, M.W.,Porta, C.,Fox, H.,Grehan, K.,Nasta, V.,Duyvesteyn, H.M.E.,De Colibus, L.,Marsian, J.,Murdoch, I.,Ponndorf, D.,Kim, S.R.,Shah, S.,Carlyle, S.,Swanson, J.J.,Matthews, S.,Nicol, C.,Lomonossoff, G.P.,Macadam, A.J.,Fry, E.E.,Stuart, D.I.,Stonehouse, N.J.,Rowlands, D.J.
Recombinant expression systems for production of stabilised virus-like particles as next-generation polio vaccines.
Nat Commun, 16:831-831, 2025

PubMed Abstract: Polioviruses have caused crippling disease in humans for centuries, prior to the successful development of vaccines in the mid-1900's, which dramatically reduced disease prevalence. Continued use of these vaccines, however, threatens ultimate disease eradication and achievement of a polio-free world. Virus-like particles (VLPs) that lack a viral genome represent a safer potential vaccine, although they require particle stabilization. Using our previously established genetic techniques to stabilize the structural capsid proteins, we demonstrate production of poliovirus VLPs of all three serotypes, from four different recombinant expression systems. We compare the antigenicity, thermostability and immunogenicity of these stabilized VLPs against the current inactivated polio vaccine, demonstrating equivalent or superior immunogenicity in female Wistar rats. Structural analyses of these recombinant VLPs provide a rational understanding of the stabilizing mutations and the role of potential excipients. Collectively, we have established these poliovirus stabilized VLPs as viable next-generation vaccine candidates for the future.

PubMed: 39827284
DOI: 10.1038/s41467-025-56118-z

実験手法

ELECTRON MICROSCOPY (3.3 Å)