9EXR

Wzc-K540M-3YE-N711Y MgADP C8

Summary for 9EXR

• Entry DOI: 10.2210/pdb9exr/pdb
• EMDB information: 50047
• Descriptor: Putative transmembrane protein Wzc, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION (3 entities in total)
• Functional Keywords: wzc-k540m-3ye-n711y, membrane protein
• Biological source: Escherichia coli
• Total number of polymer chains: 8
• Total formula weight: 647757.92

Authors

Liu, J.W.,Yang, Y.,Naismith, J.H. (deposition date: 2024-04-08, release date: 2025-04-23, Last modification date: 2025-04-30)

Primary citation

Yang, Y.,Batista, M.,Clarke, B.R.,Agyare-Tabbi, M.R.,Song, H.,Kuehfuss, N.M.,Le Bas, A.,Robinson, C.V.,Whitfield, C.,Stansfeld, P.J.,Naismith, J.H.,Liu, J.
Molecular basis for the phosphorylation of bacterial tyrosine kinase Wzc.
Nat Commun, 16:3437-3437, 2025

PubMed Abstract: The regulation of polymerisation and translocation of biomolecules is fundamental. Wzc, an integral cytoplasmic membrane tyrosine autokinase protein serves as the master regulator of the biosynthesis and export of many bacterial capsular polysaccharides and exopolysaccharides. Such polysaccharides play essential roles in infection, defence, and some are important industrial products. Wzc comprises a large periplasmic domain, two transmembrane helices and a C-terminal cytoplasmic kinase domain with a tyrosine-rich tail. Wzc regulates polymerisation functions through cycling the formation and dissociation of an octameric complex, driven by changes in the phosphorylation status of the tyrosine-rich tail. E. coli Wzc serves a model for a wider family of polysaccharide co-polymerases. Here, we determine structures of intermediate states with different extents of phosphorylation. Structural and computational data reveal the pre-ordering of the tyrosine-rich tail, the molecular basis underlying the unidirectionality of phosphorylation events, and the underlying structural dynamics on how phosphorylation status is transmitted.

PubMed: 40210632
DOI: 10.1038/s41467-025-58693-7

Experimental method

ELECTRON MICROSCOPY (2.49 Å)