9EUY

Cryo-EM structure of the full-length Pseudomonas aeruginosa bacteriophytochrome in its Pfr state

9EUY の概要

• エントリーDOI: 10.2210/pdb9euy/pdb
• EMDBエントリー: 19989
• 分子名称: Bacteriophytochrome, 3-[2-[(Z)-[3-(2-carboxyethyl)-5-[(Z)-(4-ethenyl-3-methyl-5-oxidanylidene-pyrrol-2-ylidene)methyl]-4-methyl-pyrrol-1-ium -2-ylidene]methyl]-5-[(Z)-[(3E)-3-ethylidene-4-methyl-5-oxidanylidene-pyrrolidin-2-ylidene]methyl]-4-methyl-1H-pyrrol-3- yl]propanoic acid (2 entities in total)
• 機能のキーワード: photosensor, photoreceptor, phytochrome, bacterial protein, cytosolic protein
• 由来する生物種: Pseudomonas aeruginosa
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 165361.07

構造登録者

Bodizs, S.,Westenhoff, S. (登録日: 2024-03-28, 公開日: 2024-09-04, 最終更新日: 2024-11-20)

主引用文献

Bodizs, S.,Meszaros, P.,Grunewald, L.,Takala, H.,Westenhoff, S.
Cryo-EM structures of a bathy phytochrome histidine kinase reveal a unique light-dependent activation mechanism.
Structure, 32:1952-1962.e3, 2024

PubMed Abstract: Phytochromes are photoreceptor proteins in plants, fungi, and bacteria. They can adopt two photochromic states with differential biochemical responses. The structural changes transducing the signal from the chromophore to the biochemical output modules are poorly understood due to challenges in capturing structures of the dynamic, full-length protein. Here, we present cryoelectron microscopy (cryo-EM) structures of the phytochrome from Pseudomonas aeruginosa (PaBphP) in its resting (Pfr) and photoactivated (Pr) state. The kinase-active Pr state has an asymmetric, dimeric structure, whereas the kinase-inactive Pfr state opens up. This behavior is different from other known phytochromes and we explain it with the unusually short connection between the photosensory and output modules. Multiple sequence alignment of this region suggests evolutionary optimization for different modes of signal transduction in sensor proteins. The results establish a new mechanism for light-sensing by phytochrome histidine kinases and provide input for the design of optogenetic phytochrome variants.

PubMed: 39216473
DOI: 10.1016/j.str.2024.08.008

実験手法

ELECTRON MICROSCOPY (2.95 Å)