9ETZ

III2IV respiratory supercomplex from Saccharomyces cerevisiae

9ETZ の概要

• エントリーDOI: 10.2210/pdb9etz/pdb
• EMDBエントリー: 19963
• 分子名称: Cytochrome b-c1 complex subunit 1, mitochondrial, Cytochrome b-c1 complex subunit 10, mitochondrial, Cytochrome c oxidase subunit 1, ... (36 entities in total)
• 機能のキーワード: respiration, supercomplex, membrane protein
• 由来する生物種: Saccharomyces cerevisiae (brewer's yeast); 詳細
• タンパク質・核酸の鎖数: 32
• 化学式量合計: 701790.41

構造登録者

Moe, A.,Brzezinski, P. (登録日: 2024-03-27, 公開日: 2024-10-02, 最終更新日: 2025-07-09)

主引用文献

Lobez, A.P.,Wu, F.,Di Trani, J.M.,Rubinstein, J.L.,Oliveberg, M.,Brzezinski, P.,Moe, A.
Electron transfer in the respiratory chain at low salinity.
Nat Commun, 15:8241-8241, 2024

PubMed Abstract: Recent studies have established that cellular electrostatic interactions are more influential than assumed previously. Here, we use cryo-EM and perform steady-state kinetic studies to investigate electrostatic interactions between cytochrome (cyt.) c and the complex (C) III-IV supercomplex from Saccharomyces cerevisiae at low salinity. The kinetic studies show a sharp transition with a Hill coefficient ≥2, which together with the cryo-EM data at 2.4 Å resolution indicate multiple cyt. c molecules bound along the supercomplex surface. Negatively charged loops of CIII subunits Qcr6 and Qcr9 become structured to interact with cyt. c. In addition, the higher resolution allows us to identify water molecules in proton pathways of CIV and, to the best of our knowledge, previously unresolved cardiolipin molecules. In conclusion, the lowered electrostatic screening renders engagement of multiple cyt. c molecules that are directed by electrostatically structured CIII loops to conduct electron transfer between CIII and CIV.

PubMed: 39300056
DOI: 10.1038/s41467-024-52475-3

実験手法

ELECTRON MICROSCOPY (2.4 Å)