9ETO

PsiK from Psilocybe cubensis

9ETO の概要

• エントリーDOI: 10.2210/pdb9eto/pdb
• 分子名称: 4-hydroxytryptamine kinase, DI(HYDROXYETHYL)ETHER, SULFATE ION, ... (6 entities in total)
• 機能のキーワード: kinase, transferase
• 由来する生物種: Psilocybe cubensis
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 83275.01

構造登録者

Werten, S.,Rupp, B. (登録日: 2024-03-26, 公開日: 2024-11-06, 最終更新日: 2025-02-19)

主引用文献

Rogge, K.,Wagner, T.J.,Hoffmeister, D.,Rupp, B.,Werten, S.
Substrate recognition by the 4-hydroxytryptamine kinase PsiK in psilocybin biosynthesis.
Febs Lett., 599:447-455, 2025

PubMed Abstract: Psilocybin, the natural hallucinogen from Psilocybe (magic) mushrooms, is a highly promising drug candidate for the treatment of depression and several other mental health conditions. Biosynthesis of psilocybin from the amino acid l-tryptophan involves four strictly sequential modifications. The third of these, ATP-dependent phosphorylation of the intermediate 4-hydroxytryptamine, is catalysed by PsiK. Here we present a crystallographic analysis and a structure-based mutagenesis study of this kinase, providing insight into its mode of substrate recognition. The results of our work will support future bioengineering efforts aimed at generating variants of psilocybin with enhanced therapeutic properties.

PubMed: 39449146
DOI: 10.1002/1873-3468.15042

実験手法

X-RAY DIFFRACTION (2.54 Å)