9EP9

NMR solution structure of lipid transfer protei Sola l7 from tomato seeds

9EP9 の概要

• エントリーDOI: 10.2210/pdb9ep9/pdb
• NMR情報: BMRB: 34910
• 分子名称: Non-specific lipid-transfer protein (1 entity in total)
• 機能のキーワード: lipid transfer protein fatty acids, allergen
• 由来する生物種: Solanum lycopersicum (tomato)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 9453.61

構造登録者

Parron-Ballesteros, J.,Mantin-Pedraz, L.,G.Gordo, R.,Mayorga, C.,Villaba, M.,Batanero, E.,Pantoja-Uceda, D.,Turnay, J. (登録日: 2024-03-18, 公開日: 2024-09-04, 最終更新日: 2024-10-09)

主引用文献

Parron-Ballesteros, J.,Martin-Pedraza, L.,Gordo, R.G.,Mayorga, C.,Pastor-Vargas, C.,Titaux-Delgado, G.A.,Villalba, M.,Batanero, E.,Pantoja-Uceda, D.,Turnay, J.
Long-chain fatty acids block allergic reaction against lipid transfer protein Sola l 7 from tomato seeds.
Protein Sci., 33:e5154-e5154, 2024

PubMed Abstract: Due to the benefits of tomato as an antioxidant and vitamin source, allergy to this vegetable food is a clinically concerning problem. Sola l 7, a class I lipid transfer protein found in tomato seeds, has been identified as an allergen linked to severe anaphylaxis. However, the role of lipid binding in Sola l 7-induced allergy remains unclear. Here, the three-dimensional structure of recombinant Sola l 7 (rSola l 7) has been elucidated using nuclear magnetic resonance spectroscopy (NMR). Its interaction with free fatty acids has been deeply studied; fluorescence emission spectroscopy revealed that different long-chain fatty acids interact with the protein, affecting the only tyrosine residue present in Sola l 7. On the contrary, no changes in the overall secondary structure were observed after the analysis of the circular dichroism spectra in the presence of fatty acids. Unsaturated oleic and linoleic fatty acids presented higher affinity and promoted more significant changes than saturated or short-chain fatty acids. H-N HSQC NMR spectra allowed to determine the regions of the protein that were modified when rSola l 7 interacts with the fatty acids, suggesting epitope modification after the interaction. For corroboration, IgG and IgE binding to rSola l 7 were assessed in the presence of free fatty acids, revealing that both IgE and IgG binding were significantly lower than in their absence, suggesting a potential protective role of unsaturated fatty acids in tomato allergy.

PubMed: 39180496
DOI: 10.1002/pro.5154

実験手法

SOLUTION NMR