9EOG

PHF type tau filament from R406W mutant

Summary for 9EOG

• Entry DOI: 10.2210/pdb9eog/pdb
• Related: 9EO7
• EMDB information: 19854
• Descriptor: Microtubule-associated protein tau (1 entity in total)
• Functional Keywords: tf, tau filament, r406w, protein fibril
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 6
• Total formula weight: 275519.23

Authors

Qi, C.,Scheres, S.H.W.,Michel, G. (deposition date: 2024-03-14, release date: 2024-05-29, Last modification date: 2025-03-26)

Primary citation

Qi, C.,Lovestam, S.,Murzin, A.G.,Peak-Chew, S.,Franco, C.,Bogdani, M.,Latimer, C.,Murrell, J.R.,Cullinane, P.W.,Jaunmuktane, Z.,Bird, T.D.,Ghetti, B.,Scheres, S.H.W.,Goedert, M.
Tau filaments with the Alzheimer fold in human MAPT mutants V337M and R406W.
Nat.Struct.Mol.Biol., 2025

PubMed Abstract: Frontotemporal dementia (FTD) and Alzheimer's disease (AD) are the most common forms of early-onset dementia. Unlike AD, FTD begins with behavioral changes before the development of cognitive impairment. Dominantly inherited mutations in MAPT, the microtubule-associated protein tau gene, give rise to cases of FTD and parkinsonism linked to chromosome 17. These individuals develop abundant filamentous tau inclusions in brain cells in the absence of β-amyloid deposits. Here, we used cryo-electron microscopy to determine the structures of tau filaments from the brains of human MAPT mutants V337M and R406W. Both amino acid substitutions gave rise to tau filaments with the Alzheimer fold, which consisted of paired helical filaments in all V337M and R406W cases and of straight filaments in two V337M cases. We also identified another assembly of the Alzheimer fold into triple tau filaments in a V337M case. Filaments assembled from recombinant tau (297-391) with substitution V337M had the Alzheimer fold and showed an increased rate of assembly.

PubMed: 40044789
DOI: 10.1038/s41594-025-01498-5

Experimental method

ELECTRON MICROSCOPY (3 Å)