9EK3

HIV-1 immature WT matrix protein p17 lattice

Summary for 9EK3

• Entry DOI: 10.2210/pdb9ek3/pdb
• EMDB information: 52060
• Descriptor: Matrix protein p17, MYRISTIC ACID (2 entities in total)
• Functional Keywords: matrix, hiv-1, p17, hiv-1 p17, virus, gag, structural protein, viral protein
• Biological source: Human immunodeficiency virus type 1 (HIV-1)
• Total number of polymer chains: 39
• Total formula weight: 519220.81

Authors

Rey, J.S.,Perilla, J.R.,Chen, L.,Zhang, P. (deposition date: 2024-11-30, release date: 2025-01-08, Last modification date: 2025-05-21)

Primary citation

Chen, L.,Hikichi, Y.,Rey, J.S.,Akil, C.,Zhu, Y.,Veler, H.,Shen, Y.,Perilla, J.R.,Freed, E.O.,Zhang, P.
Structural maturation of the matrix lattice is not required for HIV-1 particle infectivity.
Sci Adv, 11:eadv4356-eadv4356, 2025

PubMed Abstract: During HIV-1 maturation, the matrix (MA) lattice underlying the viral membrane undergoes a structural rearrangement, and the newly released capsid (CA) protein forms a mature CA. While it is well established that CA formation is essential for particle infectivity, the functional role of MA structural maturation remains unclear. Here, we examine maturation of an MA triple mutant, L20K/E73K/A82T, which, despite replicating similarly to wild-type (WT) in some cell lines, exhibits distinct biochemical behaviors that suggest altered MA-MA interactions. Cryo-electron tomography with subtomogram averaging reveals that, although the MA lattice in immature L20K/E73K/A82T virions closely resembles that of the WT, mature L20K/E73K/A82T virions lack a detectable MA lattice. All-atom molecular dynamics simulations suggest that this absence results from destabilized inter-trimer MA interactions in mature L20K/E73K/A82T mutant virions. These findings suggest that an ordered, membrane-associated mature MA lattice is not essential for HIV-1 infectivity, providing insights into the structural requirements for HIV-1 particle maturation and generation of infectious particles.

PubMed: 40344051
DOI: 10.1126/sciadv.adv4356

Experimental method

ELECTRON MICROSCOPY (8 Å)