9EK3

HIV-1 immature WT matrix protein p17 lattice

9EK3 の概要

• エントリーDOI: 10.2210/pdb9ek3/pdb
• EMDBエントリー: 52060
• 分子名称: Matrix protein p17, MYRISTIC ACID (2 entities in total)
• 機能のキーワード: matrix, hiv-1, p17, hiv-1 p17, virus, gag, structural protein, viral protein
• 由来する生物種: Human immunodeficiency virus type 1 (HIV-1)
• タンパク質・核酸の鎖数: 39
• 化学式量合計: 519220.81

構造登録者

Rey, J.S.,Perilla, J.R.,Chen, L.,Zhang, P. (登録日: 2024-11-30, 公開日: 2025-01-08, 最終更新日: 2025-05-21)

主引用文献

Chen, L.,Hikichi, Y.,Rey, J.S.,Akil, C.,Zhu, Y.,Veler, H.,Shen, Y.,Perilla, J.R.,Freed, E.O.,Zhang, P.
Structural maturation of the matrix lattice is not required for HIV-1 particle infectivity.
Sci Adv, 11:eadv4356-eadv4356, 2025

PubMed Abstract: During HIV-1 maturation, the matrix (MA) lattice underlying the viral membrane undergoes a structural rearrangement, and the newly released capsid (CA) protein forms a mature CA. While it is well established that CA formation is essential for particle infectivity, the functional role of MA structural maturation remains unclear. Here, we examine maturation of an MA triple mutant, L20K/E73K/A82T, which, despite replicating similarly to wild-type (WT) in some cell lines, exhibits distinct biochemical behaviors that suggest altered MA-MA interactions. Cryo-electron tomography with subtomogram averaging reveals that, although the MA lattice in immature L20K/E73K/A82T virions closely resembles that of the WT, mature L20K/E73K/A82T virions lack a detectable MA lattice. All-atom molecular dynamics simulations suggest that this absence results from destabilized inter-trimer MA interactions in mature L20K/E73K/A82T mutant virions. These findings suggest that an ordered, membrane-associated mature MA lattice is not essential for HIV-1 infectivity, providing insights into the structural requirements for HIV-1 particle maturation and generation of infectious particles.

PubMed: 40344051
DOI: 10.1126/sciadv.adv4356

実験手法

ELECTRON MICROSCOPY (8 Å)