9EGC
AclA from Tenacibaculum discolor in complex with the C8-N-acyl cyclolysine reaction product (C8-ACL)
これはPDB形式変換不可エントリーです。
9EGC の概要
エントリーDOI | 10.2210/pdb9egc/pdb |
関連するPDBエントリー | 9EGB 9EGD |
分子名称 | THIF-type NAD/FAD binding fold domain-containing protein, N-[(3R)-2-oxoazepan-3-yl]octanamide, MALEIC ACID, ... (5 entities in total) |
機能のキーワード | n-acyl-cyclolysine, biosynthesis, signaling, transferase |
由来する生物種 | Tenacibaculum discolor |
タンパク質・核酸の鎖数 | 7 |
化学式量合計 | 213517.69 |
構造登録者 | Shirkey, J.D.,Jeffrey, P.D.,Linares-Otoya, L.,Khatri Chhetri, B.,Donia, M.S.,Hughson, F.M. (登録日: 2024-11-21, 公開日: 2025-08-13, 最終更新日: 2025-09-03) |
主引用文献 | Linares-Otoya, L.,Shirkey, J.D.,Chhetri, B.K.,Mira, A.,Biswas, A.,Neff, S.L.,Linares-Otoya, M.V.,Chen, Y.,Campos-Florian, J.V.,Ganoza-Yupanqui, M.L.,Jeffrey, P.D.,Hughson, F.M.,Donia, M.S. Discovery of a widespread chemical signalling pathway in the Bacteroidota. Nature, 2025 Cited by PubMed Abstract: Considerable advances have been made in characterizing bioactive molecules secreted by bacteria, yet the regulatory elements controlling their production remain largely understudied. Here we identify and characterize the N-acyl-cyclolysine (ACL) system-a cell-density-dependent chemical signalling system specific to and widespread in the phylum Bacteroidota (formerly Bacteroidetes)-and show that it regulates the expression of co-localized operons encoding diverse secreted molecules. Using genetic and biochemical analyses, combined with structural studies of a key biosynthetic enzyme, AclA, we elucidate the molecular structure of various ACLs and their complete biosynthetic pathway involving L-lysine acylation and ATP-dependent cyclization. Furthermore, we find that secreted ACLs are sensed by a dedicated transcription factor, AclR, resulting in the expression of associated operons and the autoinduction of ACL biosynthesis. Moreover, we show that different Bacteroidota strains produce structurally diverse ACLs and encode transcription factors with varying ligand specificities. Finally, we find that the acl circuit is widely distributed and transcribed in human gut and oral microbiome samples, with clear evidence for an active role in regulating associated operons under host colonization conditions. Understanding the function of the ACL system in different contexts has the potential to reveal details about the biology, ecology and chemistry of the Bacteroidota and how members of this phylum interact with their environments and hosts. PubMed: 40836091DOI: 10.1038/s41586-025-09418-9 主引用文献が同じPDBエントリー |
実験手法 | X-RAY DIFFRACTION (2.55 Å) |
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