9EF4

Cryo-EM structure of Drosophila melanogaster insulin receptor (dmIR) bound with two DILP1, symmetric conformation

9EF4 の概要

• エントリーDOI: 10.2210/pdb9ef4/pdb
• EMDBエントリー: 47969
• 分子名称: DILP1 B-chain, DILP1 A-chain, Insulin-like receptor, ... (4 entities in total)
• 機能のキーワード: insulin receptor, dilp, structural protein
• 由来する生物種: Drosophila melanogaster (fruit fly); 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 498455.67

構造登録者

Bai, X.C. (登録日: 2024-11-19, 公開日: 2025-10-01, 最終更新日: 2025-11-12)

主引用文献

Cai, K.,Ng, M.,Yamamoto, R.R.,Hossain, M.A.,Hall, C.,Wade, J.D.,Tatar, M.,Choi, E.,Bai, X.C.
Structure and activation of the Drosophila insulin receptor by three Drosophila insulin-like peptides.
Nat Commun, 16:9504-9504, 2025

PubMed Abstract: Insulin/IGF signaling (IIS) is a highly conserved pathway essential for physiological regulation from yeast to mammals. In Drosophila melanogaster, a single insulin-like receptor (dmIR) interacts with various insulin-like peptides (DILPs), leading to diverse signaling and functional outcomes. However, the mechanisms by which different DILPs result in varied receptor activation and biological responses remain unclear. Here, we determine the cryo-electron microscopy (cryo-EM) structures of dmIR in complex with three DILPs: DILP1, DILP2, and DILP5. Our structural analyses reveal that each DILP induces distinct conformations of dmIR: the dmIR/DILP5 complex adopts the Ƭ-shaped asymmetric conformation with three bound DILP5 molecules; the dmIR/DILP2 complex displays the Γ-shaped asymmetric conformation with a single bound DILP2 molecule; and the dmIR/DILP1 complex shows both a Γ-shaped asymmetric conformation and a symmetric conformation that resembles a T-shape with a splayed stem. Functional assays demonstrate that the efficacy of DILP-mediated dmIR activation differs, with DILP5 inducing higher levels of receptor autophosphorylation, followed by DILP2 and DILP1. Together, these findings suggest that the distinct interactions between dmIR and DILPs dictate specific patterns of receptor activation.

PubMed: 41152236
DOI: 10.1038/s41467-025-64586-6

実験手法

ELECTRON MICROSCOPY (3.9 Å)